Gallo Giovanni, Zannini Domenico, Immirzi Barbara, De Bruno Alessandra, Fiorentino Gabriella, Dal Poggetto Giovanni
Department of Biology, University of Naples Federico II, Complesso Universitario di Monte Sant'Angelo, 80126 Napoli, Italy.
Division of Microbiology, Faculty of Biology, Ludwig-Maximilians-Universität München, 82152 Martinsried, Germany.
Antioxidants (Basel). 2023 Mar 21;12(3):763. doi: 10.3390/antiox12030763.
The aim of this work was to exploit the antioxidant potential of molecules recovered during the pectin purification process of waste and to encapsulate them in stable pectin films, with a view to a green and circular economy process. Antioxidant molecules were recovered during the pectin purification process, further recovering matter from the waste. Seven molecules were identified and quantified, and the antioxidant power of the mixture and its stability over time was evaluated. To improve the stability of the bioactive fraction, this was complexed with 2-hydroxypropyl-β-cyclodextrin (HP-β-CD); indeed, this procedure increased their thermal stability from 120 °C up to 250 °C, as verified by thermogravimetry. Furthermore, the most promising complexes were studied under autoclave-like conditions (120 °C, 28 min) to simulate thermal sterilization. The antioxidants and HP-β-CD were combined in a pectin film, showing increased stability over time (up to three times) compared to uncomplexed antioxidants. This process represents a first step towards the development of applicable devices for the delivery of antioxidant molecules.
这项工作的目的是利用在废弃物果胶纯化过程中回收的分子的抗氧化潜力，并将其封装在稳定的果胶薄膜中，以实现绿色循环经济过程。在果胶纯化过程中回收了抗氧化分子，进一步从废弃物中回收物质。鉴定并定量了七种分子，并评估了混合物的抗氧化能力及其随时间的稳定性。为提高生物活性部分的稳定性，将其与2-羟丙基-β-环糊精（HP-β-CD）络合；事实上，通过热重分析证实，该方法将其热稳定性从120℃提高到了250℃。此外，在类似高压灭菌的条件（120℃，28分钟）下研究了最有前景的络合物，以模拟热灭菌。抗氧化剂和HP-β-CD被结合在果胶薄膜中，与未络合的抗氧化剂相比，其随时间的稳定性提高了（高达三倍）。这一过程代表了朝着开发用于递送抗氧化分子的适用装置迈出的第一步。
