Mohammad Shuhiba, Bhattacharjee Jayonta, Tzaneva Velislava, Hutchinson Kelly Ann, Shaikh Madeeha, Fernandes da Silva Danilo, Burger Dylan, Adamo Kristi B
School of Human Kinetics, Faculty of Health Sciences, University of Ottawa, Ottawa, ON K1N 6N5, Canada.
Kidney Research Centre, Department of Cellular and Molecular Medicine, The Ottawa Hospital Research Institute, University of Ottawa, Ottawa, ON K1H 8L6, Canada.
Biomedicines. 2023 Mar 11;11(3):857. doi: 10.3390/biomedicines11030857.
Exercise induces the release of small extracellular vesicles (sEVs) into circulation that are postulated to mediate tissue cross-talk during exercise. We previously reported that pregnant individuals released greater levels of sEVs into circulation after exercise compared to matched non-pregnant controls, but their biological functions remain unknown. In this study, sEVs isolated from the plasma of healthy pregnant and non-pregnant participants after a single bout of moderate-intensity exercise were evaluated for their impact on trophoblasts . Exercise-associated sEVs were found localized within the cytoplasm of BeWo choriocarcinoma cells, used to model trophoblasts in vitro. Exposure to exercise-associated sEVs did not significantly alter BeWo cell proliferation, gene expression of angiogenic growth factors and , or the release of the hormone human chorionic gonadotropin. The results from this pilot study support that exercise-associated sEVs could interact with trophoblasts in vitro, and warrant further investigation to reveal their potential role in communicating the effects of exercise to the maternal-fetal interface.
运动可促使小细胞外囊泡（sEVs）释放进入循环系统，据推测这些小囊泡在运动过程中介导组织间的相互作用。我们之前报道过，与相匹配的非孕期对照相比，孕妇在运动后会向循环系统中释放更高水平的sEVs，但其生物学功能仍不清楚。在本研究中，我们评估了单次中等强度运动后从健康孕妇和非孕妇参与者血浆中分离出的sEVs对滋养层细胞的影响。运动相关的sEVs被发现定位于体外用于模拟滋养层细胞的BeWo绒毛膜癌细胞的细胞质内。暴露于运动相关的sEVs并未显著改变BeWo细胞的增殖、血管生成生长因子和的基因表达，也未改变激素人绒毛膜促性腺激素的释放。这项初步研究的结果支持运动相关的sEVs可在体外与滋养层细胞相互作用，有必要进一步研究以揭示它们在将运动效应传递至母胎界面方面的潜在作用。
