Sonnenfeld G, Hudgens R W
Cancer Res. 1986 Jun;46(6):2779-83.
A peristaltic pump smoking machine that allows simultaneous generation of mainstream (active) and sidestream (passive) smoke from a cigarette was used to expose cultures of murine L-929 cells, a potent producer of interferon, to smoke. The cigarette used was the University of Kentucky 2R1 reference cigarette. The dosages of smoke used for exposure were the highest doses possible that generated a minimum toxic effect, and they were serially diluted to lower doses. Dosages were determined by the number of smoke puffs generated, the volume of smoke puffs generated, and the total particulate matter deposited on Cambridge filters in the smoke machine. Viability of exposed cells was equivalent to control cell cultures. Interferon-alpha/beta was induced by addition of polyriboinosinic-polyribocytidylic acid to the cells. Interferon production was substantially reduced in viable cells exposed to mainstream or sidestream smoke. Aging of smoke by delaying time of exposure of the cells to the smoke, or filtration of smoke through activated charcoal substantially decreased the alteration of interferon production by smoke exposure. These results suggest that actual exposure of cells to mainstream or sidestream smoke can inhibit in vitro interferon-alpha/beta induction, but the cells can be protected from these effects by smoke manipulation.
一种蠕动泵式吸烟机可使香烟同时产生主流（主动）烟雾和侧流（被动）烟雾，利用该吸烟机使强效干扰素产生源——小鼠L - 929细胞培养物暴露于烟雾中。所使用的香烟为肯塔基大学2R1参考香烟。用于暴露的烟雾剂量是产生最小毒性效应的最高剂量，并依次稀释至较低剂量。剂量由产生的烟雾抽吸次数、产生的烟雾抽吸体积以及沉积在吸烟机中剑桥滤片上的总颗粒物确定。暴露细胞的活力与对照细胞培养物相当。通过向细胞中添加聚肌苷酸 - 聚胞苷酸来诱导α/β干扰素。暴露于主流或侧流烟雾的活细胞中干扰素的产生显著减少。通过延迟细胞暴露于烟雾的时间来使烟雾老化，或使烟雾通过活性炭过滤，可大幅降低烟雾暴露对干扰素产生的改变。这些结果表明，细胞实际暴露于主流或侧流烟雾可抑制体外α/β干扰素的诱导，但通过烟雾处理可保护细胞免受这些影响。
