Sonnenfeld G, Hudgens R W
Cancer Res. 1983 Oct;43(10):4720-2.
Mice were treated with several different potentially carcinogenic components of tobacco smoke. The chemicals used were: 4-aminobiphenyl and aniline-HCl, which are found in high concentrations in sidestream tobacco smoke; hydrazine sulfate, which is found in high concentrations in mainstream tobacco smoke; and 2-methylquinoline, which is found in intermediate concentrations in both sidestream and mainstream smoke. The chemicals were injected i.p. into mice, and then alpha/beta interferon was induced in the mice by i.v. injection of polyriboinosinic-polyribocytidylic acid. The interferon was induced either 2, 24, or 48 hr after treatment with the tobacco smoke components. Mice treated with 4-aminobiphenyl showed some depression of interferon production 2 hr after treatment, maximum inhibition of interferon induction 24 hr after treatment, and a return to control levels of interferon 48 hr after treatment. Mice treated with hydrazine sulfate showed maximum inhibition of interferon induction 24 hr after treatment but no effects at any other treatment time. These components were the most carcinogenic chemicals of those utilized in this study. Treatment of mice with aniline-HCl, a chemical whose carcinogenic potential is still debated, resulted in marginal depression of interferon induction 24 hr after treatment. 2-Methylquinoline, the chemical with the lowest carcinogenic potential in this study, had no effect on interferon induction after administration to mice. In vivo interferon induction was, therefore, inhibited by treatment of mice with chemical carcinogens found in tobacco smoke. The efficacy of the chemical in inhibiting interferon induction was not influenced by the mainstream or sidestream smoke predominance of the chemical.
用烟草烟雾中几种不同的潜在致癌成分处理小鼠。所使用的化学物质有：4-氨基联苯和盐酸苯胺，它们在侧流烟草烟雾中含量很高；硫酸肼，它在主流烟草烟雾中含量很高；以及2-甲基喹啉，它在侧流和主流烟雾中的含量处于中等水平。将这些化学物质腹腔注射到小鼠体内，然后通过静脉注射聚肌苷酸-聚胞苷酸在小鼠体内诱导α/β干扰素。在使用烟草烟雾成分处理后2、24或48小时诱导干扰素。用4-氨基联苯处理的小鼠在处理后2小时显示出干扰素产生略有降低，在处理后24小时干扰素诱导受到最大抑制，并且在处理后48小时恢复到干扰素的对照水平。用硫酸肼处理的小鼠在处理后24小时显示出干扰素诱导受到最大抑制，但在任何其他处理时间均无影响。这些成分是本研究中使用的最具致癌性的化学物质。用盐酸苯胺处理小鼠（一种致癌潜力仍存在争议的化学物质），在处理后24小时导致干扰素诱导略有降低。2-甲基喹啉，本研究中致癌潜力最低的化学物质，在给予小鼠后对干扰素诱导没有影响。因此，用烟草烟雾中发现的化学致癌物处理小鼠会抑制体内干扰素的诱导。化学物质在抑制干扰素诱导方面的功效不受该化学物质在主流或侧流烟雾中占优势的影响。
