幽门螺杆菌毒力因子细胞毒素相关基因 A（CagA）诱导冠状动脉平滑肌细胞血管钙化。

Helicobacter Pylori Virulence Factor Cytotoxin-Associated Gene A (CagA) Induces Vascular Calcification in Coronary Artery Smooth Muscle Cells.

机构信息

Department of Clinical Science and Education, Division of Cardiology, Karolinska Institutet, Södersjukhuset, SE-118 83 Stockholm, Sweden.

Department of Cardiology, Södersjukhuset, SE-118 83 Stockholm, Sweden.

出版信息

Int J Mol Sci. 2023 Mar 11;24(6):5392. doi: 10.3390/ijms24065392.

DOI:10.3390/ijms24065392

PMID:36982467

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10049385/

Abstract

() has been associated with cardiovascular diseases. The pro-inflammatory virulence factor cytotoxin-associated gene A (CagA) has been detected in serum exosomes of -infected subjects and may exert systemic effects throughout the cardiovascular system. The role of and CagA in vascular calcification was hitherto unknown. The aim of this study was to determine the vascular effects of CagA through human coronary artery smooth muscle cell (CASMC) osteogenic and pro-inflammatory effector gene expression as well as interleukin 1β secretion and cellular calcification. CagA upregulated bone morphogenic protein 2 (BMP-2) associated with an osteogenic CASMC phenotype switch and induced increased cellular calcification. Furthermore, a pro-inflammatory response was observed. These results support that may contribute to vascular calcification through CagA rendering CASMCs osteogenic and inducing calcification.

摘要

() 与心血管疾病有关。已在感染的受试者的血清外泌体中检测到促炎毒力因子细胞毒素相关基因 A (CagA)，它可能对整个心血管系统产生全身性影响。和 CagA 在血管钙化中的作用尚不清楚。本研究旨在通过人冠状动脉平滑肌细胞 (CASMC) 的成骨和成炎效应基因表达以及白细胞介素 1β 分泌和细胞钙化来确定 CagA 的血管作用。CagA 上调骨形态发生蛋白 2 (BMP-2)，与成骨 CASMC 表型转换相关，并诱导细胞钙化增加。此外，还观察到促炎反应。这些结果表明，通过使 CASMC 成骨并诱导钙化，可能有助于血管钙化。