The Invasive Anemone sp. of the Coral Reef as a Source of Sulfur- and Nitrogen-Containing Metabolites and Cytotoxic 5,8-Epidioxy Steroids.

The -genus anemones were examined for their proteinaceous poisons over 50 years ago. On the other hand, the current research focuses on isolating and describing the non-proteinaceous secondary metabolites from the invasive anemones, which help take advantage of their population outbreak as a new source of chemical candidates and potential drug leads. From an organic extract of sp., a 1,2,4-thiadiazole-based alkaloid, identified as 3,5-bis(3-pyridinyl)-1,2,4-thiadiazole (), was found to be a new natural alkaloid despite being previously synthesized. The full assignment of NMR data of compound , based on the analysis of 2D NMR correlations, is reported herein for the first time. The proposed biosynthetic precursor thionicotinamide () was also isolated for the first time from nature along with nicotinamide (), uridine (), hypoxanthine (), and four 5,8-epidioxysteroids (-). A major secondary metabolite (-)-betonicine () was isolated from sp. and found for the first time in marine invertebrates. The four 5,8-epidioxysteroids, among other metabolites, exhibited cytotoxicity (IC 3.5-9.0 μg/mL) toward five cancer cell lines.