Hubei Key Laboratory of Radiation Chemistry and Functional Materials, Non-Power Nuclear Technology Collaborative Innovation Center, Hubei University of Science and Technology, Xianning 437100, China.
School of Pharmacy, Hubei University of Science and Technology, Xianning 437100, China.
Molecules. 2023 Mar 16;28(6):2687. doi: 10.3390/molecules28062687.
Dopamine (DA) is an important neurotransmitter. Abnormal concentration of DA can result in many neurological diseases. Developing reliable determination methods for DA is of great significance for the diagnosis and monitoring of neurological diseases. Here, a novel and simple electrochemical sensing platform for quantitative analysis of DA was constructed based on the Cu-TCPP/graphene composite (TCPP: Tetrakis(4-carboxyphenyl)porphyrin). Cu-TCPP frameworks were selected in consideration of their good electrochemical sensing potential. The graphene nanosheets with excellent conductivity were then added to further improve the sensing efficiency and stability of Cu-TCPP frameworks. The electrochemical properties of the Cu-TCPP/graphene composite were characterized, showing its large electrode active area, fast electron transfer, and good sensing performance toward DA. The signal enhancement mechanism of DA was explored. Strong accumulation ability and high electrocatalytic rate were observed on the surface of Cu-TCPP/graphene-modified glassy carbon electrode (Cu-TCPP/graphene/GCE). Based on the synergistic sensitization effect, an ultrasensitive and simple DA electrochemical sensor was developed. The linear range is 0.02-100 and 100-1000 µM, and the detection limit is 3.6 nM for the first linear range. It was also successfully used in detecting DA in serum samples, and a satisfactory recovery was obtained.
多巴胺（DA）是一种重要的神经递质。DA 浓度异常会导致许多神经疾病。开发可靠的 DA 测定方法对于神经疾病的诊断和监测具有重要意义。本工作基于 Cu-TCPP/石墨烯复合材料（TCPP：四（4-羧基苯基）卟啉）构建了一种用于定量分析 DA 的新型简单电化学传感平台。选择 Cu-TCPP 框架是考虑到其良好的电化学传感潜力。然后添加具有优异导电性的石墨烯纳米片，以进一步提高 Cu-TCPP 框架的传感效率和稳定性。对 Cu-TCPP/石墨烯复合材料的电化学性能进行了表征，结果表明其具有较大的电极活性面积、快速的电子转移和对 DA 的良好传感性能。探讨了 DA 的信号增强机制。在 Cu-TCPP/石墨烯修饰的玻碳电极（Cu-TCPP/石墨烯/GCE）表面观察到强的积累能力和高的电催化速率。基于协同敏化效应，开发了一种超灵敏和简单的 DA 电化学传感器。第一个线性范围的线性范围为 0.02-100 和 100-1000 µM，检测限为 3.6 nM。该方法还成功用于血清样品中 DA 的检测，获得了令人满意的回收率。
