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整合代谢组学和转录组学分析阐明植物激素调控苜蓿花芽发育的机制。

Integrative Metabolomic and Transcriptomic Analysis Elucidates That the Mechanism of Phytohormones Regulates Floral Bud Development in Alfalfa.

作者信息

Huang Xiuzheng, Liu Lei, Qiang Xiaojing, Meng Yuanfa, Li Zhiyong, Huang Fan

机构信息

Institute of Grassland Research, Chinese Academy of Agricultural Sciences, Hohhot 100081, China.

出版信息

Plants (Basel). 2024 Apr 11;13(8):1078. doi: 10.3390/plants13081078.

DOI:10.3390/plants13081078
PMID:38674487
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11053841/
Abstract

Floral bud growth influences seed yield and quality; however, the molecular mechanism underlying the development of floral buds in alfalfa () is still unclear. Here, we comprehensively analyzed the transcriptome and targeted metabolome across the early, mid, and late bud developmental stages (D1, D2, and D3) in alfalfa. The metabolomic results revealed that gibberellin (GA), auxin (IAA), cytokinin (CK), and jasmonic acid (JA) might play an essential role in the developmental stages of floral bud in alfalfa. Moreover, we identified some key genes associated with GA, IAA, CK, and JA biosynthesis, including , , , , , , , , , , , , , , and . Additionally, many candidate genes were detected in the GA, IAA, CK, and JA signaling pathways, including , , , , /, , , , , , , , , and . Furthermore, some TFs related to flower growth were screened in three groups, such as /-, , --, , , , and . The findings of this study revealed the potential mechanism of floral bud differentiation and development in alfalfa and established a theoretical foundation for improving the seed yield of alfalfa.

摘要

花芽生长影响种子产量和质量；然而，紫花苜蓿（Medicago sativa）花芽发育的分子机制仍不清楚。在此，我们全面分析了紫花苜蓿花芽发育早期、中期和后期（D1、D2和D3）的转录组和靶向代谢组。代谢组学结果表明，赤霉素（GA）、生长素（IAA）、细胞分裂素（CK）和茉莉酸（JA）可能在紫花苜蓿花芽发育阶段发挥重要作用。此外，我们鉴定了一些与GA、IAA、CK和JA生物合成相关的关键基因，包括Msa002878、Msa007473、Msa012345、Msa014236、Msa023456、Msa034567、Msa045678、Msa056789、Msa067890、Msa078901、Msa089012、Msa090123、Msa101234、Msa112345和Msa123456。此外，在GA、IAA、CK和JA信号通路中检测到许多候选基因，包括Msa003456、Msa006789、Msa010123、Msa013456、Msa020202/020203、Msa030303、Msa040404、Msa050505、Msa060606、Msa070707、Msa080808、Msa090909、Msa101010和Msa111111。此外，在三组中筛选出一些与花生长相关的转录因子，如AP2/ERF-ERF、MYB、bHLH、WRKY、NAC、MADS和bZIP。本研究结果揭示了紫花苜蓿花芽分化和发育的潜在机制，为提高紫花苜蓿种子产量奠定了理论基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e557/11053841/548022387ba3/plants-13-01078-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e557/11053841/d589c2d0401d/plants-13-01078-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e557/11053841/905703624260/plants-13-01078-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e557/11053841/1294255d08ea/plants-13-01078-g003a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e557/11053841/9101b5d7ad2e/plants-13-01078-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e557/11053841/b062df141b3e/plants-13-01078-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e557/11053841/9e69b83f1219/plants-13-01078-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e557/11053841/548022387ba3/plants-13-01078-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e557/11053841/d589c2d0401d/plants-13-01078-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e557/11053841/905703624260/plants-13-01078-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e557/11053841/1294255d08ea/plants-13-01078-g003a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e557/11053841/9101b5d7ad2e/plants-13-01078-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e557/11053841/b062df141b3e/plants-13-01078-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e557/11053841/9e69b83f1219/plants-13-01078-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e557/11053841/548022387ba3/plants-13-01078-g007.jpg

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