Faculty of Science and Technology, ICFAI University, Kamalghat, Agartala, Tripura, 799210, India.
Faculty of Engineering & Technology, Siksha 'O' Anusandhan (Deemed to be University), Bhubaneswar, Odisha, 751030, India.
J Membr Biol. 2023 Jun;256(3):243-255. doi: 10.1007/s00232-023-00283-z. Epub 2023 Mar 29.
Electroporation method is a useful tool for delivering drugs into various diseased tissues in the human body. As a result of an applied electric field, drug particles enter the intracellular compartment through the temporarily permeabilized cell membrane. Consequently, electroporation method allows better penetration of the drug into the diseased tissue and improves treatment clinically. In this study, a more generalized model of drug transport in a single cell is proposed. The model is able to capture non-homogeneous drug transport in the cell due to non-uniform cell membrane permeabilization. Several numerical experiments are conducted to understand the effects of electric field and drug permeability on drug uptake into the cell. Through investigation, the appropriate electric field and drug permeability are identified, which lead to sufficient drug uptake into the cell. This model can be used by experimentalists to get information prior to conduct any experiment, and it may help reduce the number of actual experiments that might be conducted otherwise.
电穿孔法是一种将药物递送至人体各种病变组织的有用工具。由于施加了电场，药物颗粒通过暂时穿孔的细胞膜进入细胞内隔室。因此，电穿孔法可以使药物更好地渗透到病变组织中，并改善临床治疗效果。在这项研究中，提出了一种更通用的单细胞药物传输模型。该模型能够捕获由于细胞膜不均匀通透性而导致的细胞内药物传输的非均相性。进行了几个数值实验来了解电场和药物渗透性对药物摄取进入细胞的影响。通过研究，确定了合适的电场和药物渗透性，从而使药物能够充分摄取进入细胞。该模型可被实验人员用于在进行任何实验之前获取信息，并且可以帮助减少可能需要进行的实际实验数量。
