Department of Surgery, University of Pécs, Pécs, Hungary.
Department of Biophysics, University of Pécs, Pécs, Hungary.
J Fluoresc. 2023 Sep;33(5):2099-2103. doi: 10.1007/s10895-023-03224-0. Epub 2023 Mar 29.
Indocyanine green is a fluorescent dye, the use of which is becoming more and more widespread in different areas of surgery. Several international studies deal with the dye's usefulness in intraoperative angiography, the localization of tumors, the more precise identification of anatomical structures, the detection of lymph nodes and lymph ducts, etc. The application of the dye is safe, but a suitable equipment park is required for its use, which entails relatively high costs.
The aim of our research is to create a detector system on a low budget, to be used safely in everyday practice and to illustrate its operation with practical examples at our own institute.
By modifying a web camera, using filter lenses and special LEDs, we created a device suitable for exciting and detecting indocyanine green fluorescence. We prove its excellent versatility during the following procedures at our institute: breast tumor surgery, kidney transplantation, bowel resection, parathyroid surgery and liver tumor resection.
The finished camera has an LED light source with a peak wavelength of 780 nm, and the incoming light is filtered by a bandpass filter with a center wavelength of 832 nm. A low budget ($112), easy-to-use tool was created, which is suitable for taking advantage of the opportunities provided by indocyanine green.
吲哚菁绿是一种荧光染料,其在不同外科领域的应用越来越广泛。多项国际研究涉及该染料在术中血管造影、肿瘤定位、更精确地识别解剖结构、淋巴结和淋巴管检测等方面的用途。该染料的应用是安全的,但需要合适的设备才能使用,这需要相对较高的成本。
我们的研究旨在创建一个低成本的探测器系统,使其在日常实践中安全使用,并在我们自己的研究所中用实际例子来说明其操作。
通过修改网络摄像头,使用滤光片和特殊的 LED,我们创建了一个适合激发和检测吲哚菁绿荧光的设备。我们在研究所的以下程序中证明了其出色的多功能性:乳腺肿瘤手术、肾移植、肠道切除术、甲状旁腺手术和肝肿瘤切除术。
成品摄像头有一个峰值波长为 780nm 的 LED 光源,输入的光通过中心波长为 832nm 的带通滤波器进行过滤。我们创建了一个低成本(112 美元)、易于使用的工具,可充分利用吲哚菁绿带来的机会。