Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, UK.
Sci Adv. 2023 Mar 29;9(13):eade9756. doi: 10.1126/sciadv.ade9756.
C photosynthesis has evolved by repurposing enzymes found in C plants. Compared with the ancestral C state, accumulation of C cycle proteins is enhanced. We used de-etiolation of C and C to understand this process. C gene expression and chloroplast biogenesis in were tightly coordinated. Although C and C photosynthesis genes showed similar induction patterns, in , C genes were more strongly induced than orthologs from . In vivo binding of TGA and homeodomain as well as light-responsive elements such as G- and I-box motifs were associated with the rapid increase in transcripts of C genes. Deletion analysis confirmed that regions containing G- and I-boxes were necessary for high expression. The data support a model in which accumulation of transcripts derived from C photosynthesis genes in C leaves is enhanced because modifications in cis allowed integration into ancestral transcriptional networks.
C 光合作用是通过重新利用 C 植物中的酶来进化的。与祖先进化的 C 状态相比，C 循环蛋白的积累得到了增强。我们使用 C 和 C 去黄化来理解这个过程。C 的基因表达和叶绿体生物发生在 中是紧密协调的。虽然 C 和 C 光合作用基因表现出相似的诱导模式，但在 中，C 基因的诱导强度比来自 的同源物更强。体内 TGA 和同源域以及光响应元件如 G-和 I-盒基序的结合与 C 基因转录本的快速增加有关。删除分析证实，含有 G-和 I-盒的区域对于高表达是必需的。这些数据支持这样一种模型，即在 C 叶中 C 光合作用基因的转录本的积累增强，是因为顺式修饰允许其整合到祖先的转录网络中。
