Zhao Lili, Huang Weixiao, Yi Sheng
Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-Innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu, China.
NMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products, Nantong University, Nantong, Jiangsu, China.
Front Neurosci. 2023 Mar 14;17:1098612. doi: 10.3389/fnins.2023.1098612. eCollection 2023.
Single-cell RNA sequencing allows the division of cell populations, offers precise transcriptional profiling of individual cells, and fundamentally advances the comprehension of cellular diversity. In the peripheral nervous system (PNS), the application of single-cell RNA sequencing identifies multiple types of cells, including neurons, glial cells, ependymal cells, immune cells, and vascular cells. Sub-types of neurons and glial cells have further been recognized in nerve tissues, especially tissues in different physiological and pathological states. In the current article, we compile the heterogeneities of cells that have been reported in the PNS and describe cellular variability during development and regeneration. The discovery of the architecture of peripheral nerves benefits the understanding of the cellular complexity of the PNS and provides a considerable cellular basis for future genetic manipulation.
单细胞RNA测序能够划分细胞群体，提供单个细胞精确的转录谱分析，并从根本上推动对细胞多样性的理解。在周围神经系统（PNS）中，单细胞RNA测序的应用识别出多种类型的细胞，包括神经元、神经胶质细胞、室管膜细胞、免疫细胞和血管细胞。在神经组织中，尤其是处于不同生理和病理状态的组织中，神经元和神经胶质细胞的亚型得到了进一步的确认。在本文中，我们汇总了周围神经系统中已报道的细胞异质性，并描述了发育和再生过程中的细胞变异性。周围神经结构的发现有助于理解周围神经系统的细胞复杂性，并为未来的基因操作提供了重要的细胞基础。
