通过组织培养对用于艰难梭菌细胞毒素常规检测的商用试剂盒的评估。
Evaluation of a commercial kit for the routine detection of Clostridium difficile cytotoxin by tissue culture.
作者信息
Wu T C, Gersch S M
出版信息
J Clin Microbiol. 1986 Apr;23(4):792-3. doi: 10.1128/jcm.23.4.792-793.1986.
Abstract
The Toxi-titer microtiter plate system (Bartels Immunodiagnostic Supplies, Inc., Bellevue, Wash.) is a simplified procedure for detecting the cytotoxin produced by Clostridium difficile in stool filtrates. In a parallel study of 74 stool specimens, results from the Toxin-titer system compared favorably with those from the conventional system. Our experience with the Toxin-titer system in testing 540 stool specimens was, in general, satisfactory, although a few problems with toxin control did occur. The method requires no specialized experience in tissue culture techniques and can therefore be adopted for routine use in clinical laboratories.
摘要
Toxi-titer微量滴定板系统（巴特尔免疫诊断用品公司，华盛顿州贝尔维尤）是一种用于检测艰难梭菌在粪便滤液中产生的细胞毒素的简化程序。在一项对74份粪便标本的平行研究中，Toxin-titer系统的结果与传统系统的结果相比具有优势。我们使用Toxin-titer系统检测540份粪便标本的经验总体上是令人满意的，尽管在毒素控制方面确实出现了一些问题。该方法不需要组织培养技术方面的专业经验，因此可用于临床实验室的常规检测。
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