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临床标本中对流免疫电泳用于艰难梭菌相关性结肠炎诊断的实用性评估。

Evaluation of the usefulness of counterimmunoelectrophoresis for diagnosis of Clostridium difficile-associated colitis in clinical specimens.

作者信息

Wu T C, Fung J C

出版信息

J Clin Microbiol. 1983 Apr;17(4):610-3. doi: 10.1128/jcm.17.4.610-613.1983.

DOI:10.1128/jcm.17.4.610-613.1983
PMID:6853688
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC272702/
Abstract

Results of counterimmunoelectrophoresis (CIE) were compared with those of isolation of Clostridium difficile and assay for cytotoxicity in HeLa cells. On the basis of 471 stool specimens, CIE exhibited a sensitivity of 38% and a specificity of 88% as compared with the cytotoxin assay. The predictive value of a reactive CIE results is low (17%), whereas the predictive value of a nonreactive CIE result is significant (96%) and therefore warrants its use as a screening test. In addition, stool filtrates may nonspecifically precipitate with the C. difficile antitoxin in the CIE test. Such nonspecific reactions may be identified by simultaneous electrophoresis against nonimmune serum.

摘要

将对流免疫电泳（CIE）的结果与艰难梭菌分离结果以及HeLa细胞细胞毒性测定结果进行了比较。基于471份粪便标本，与细胞毒素测定相比，CIE的灵敏度为38%，特异性为88%。CIE反应性结果的预测价值较低（17%），而非反应性CIE结果的预测价值较高（96%），因此有理由将其用作筛查试验。此外，粪便滤液在CIE试验中可能会与艰难梭菌抗毒素发生非特异性沉淀。这种非特异性反应可通过与非免疫血清同时电泳来识别。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da7b/272702/3e130b545325/jcm00141-0065-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da7b/272702/3e130b545325/jcm00141-0065-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da7b/272702/3e130b545325/jcm00141-0065-a.jpg

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本文引用的文献

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Comparison of two toxins produced by Clostridium difficile.艰难梭菌产生的两种毒素的比较。
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通过直接气相色谱法检测粪便中的艰难梭菌。
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