Pyziel-Serafin Anna Maria, Vetter Wiktoria, Klich Daniel, Anusz Krzysztof
Department of Food Hygiene and Public Health Protection, Institute of Veterinary Medicine, Warsaw University of Life Sciences, 02-776 Warsaw, Poland.
Department of Animal Genetics and Conservation, Institute of Animal Sciences, Warsaw University of Life Sciences, 02-787 Warsaw, Poland.
J Vet Res. 2023 Mar 17;67(1):87-92. doi: 10.2478/jvetres-2023-0015. eCollection 2023 Mar.
Among large wild game in Poland, the most numerous cervids are red deer and roe deer. Although these species live free, they should be under veterinary supervision because they can transmit infectious agents and parasites to livestock. The aim of this study was to evaluate the biodiversity of the abomasal nematodes which parasitise cervids and present the visual and dimensional characteristics of their spicules.
Overall, 2,067 spicules of nematodes derived from nine red deer and five roe deer were measured and microphotographed in order to determine the species. The predominant was additionally confirmed molecularly by PCR. The spicule lengths of the most common species found in both hosts simultaneously were compared.
Fourteen species of abomasal nematode were identified. All examined animals but one were infected. The most prevalent parasites in both host species were and . The alien was found in both hosts, whereas was identified only in red deer. was noted in red deer for the first time. A 262-base-pair nucleotide sequence of was obtained and deposited in GenBank. Significantly longer spicules were found in red deer-derived and and shorter structures were seen in .
The widespread exchange of abomasal nematodes between various ruminant species questions the relevance of their division into specialists and generalists.
在波兰的大型野生动物中，数量最多的鹿科动物是马鹿和狍。尽管这些物种自由生活，但它们应接受兽医监督，因为它们可能将传染原和寄生虫传播给家畜。本研究的目的是评估寄生于鹿科动物的皱胃线虫的生物多样性，并呈现其交合刺的视觉和尺寸特征。
总共测量并显微拍摄了来自9只马鹿和5只狍的2067条线虫交合刺，以确定其种类。优势种还通过PCR进行了分子确认。比较了在两种宿主中同时发现的最常见物种的交合刺长度。
鉴定出14种皱胃线虫。除一只动物外，所有检查的动物均被感染。两种宿主物种中最普遍的寄生虫是[具体寄生虫名称1]和[具体寄生虫名称2]。外来种[具体寄生虫名称3]在两种宿主中均被发现，而[具体寄生虫名称4]仅在马鹿中被鉴定出。[具体寄生虫名称5]首次在马鹿中被发现。获得了[具体寄生虫名称5]的一段262个碱基对的核苷酸序列并提交至GenBank。在源自马鹿的[具体寄生虫名称6]和[具体寄生虫名称7]中发现交合刺明显更长，而在[具体寄生虫名称8]中则观察到较短的结构。
各种反刍动物物种之间皱胃线虫的广泛交换对将它们分为专性寄生虫和兼性寄生虫的相关性提出了质疑。
