网络药理学与实验验证揭示苦参碱抗猪繁殖与呼吸综合征病毒的靶点
Network pharmacology and experimental validation to reveal the target of matrine against PRRSV.
作者信息
Zhao Yaxiang, Ling Xiaoya, Zhang Hua, Sun Panpan, Sun Yaogui, Yin Wei, Fan Kuohai, Yang Huizhen, Zhong Jia, Zhang Zhenbiao, Wang Jianzhong, Li Hongquan, Sun Na
机构信息
Shanxi Key Laboratory for Modernization of TCVM, College of Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi 030801, China.
Laboratory Animal Center, Shanxi Agricultural University, Taigu, Shanxi 030801, China.
出版信息
iScience. 2023 Mar 10;26(4):106371. doi: 10.1016/j.isci.2023.106371. eCollection 2023 Apr 21.
Porcine reproductive and respiratory syndrome (PRRS) is an epidemic animal infectious disease worldwide. In our previous research it was suggested that matrine could inhibit PRRSV infection both and , but the antiviral mechanisms are still undecided. Network pharmacology can well solve the difficult problem of "multiple targets, multiple pathways" in the research of TCM action targets. The results of network pharmacology indicated that matrine exerts its anti-PRRSV effect by targeting HSPA8 and HSP90AB1. The results of real-time fluorescent quantitative PCR and western blot showed that infection with PRRSV induced a significant increase in the expression of HSPA8 and HSP90AB1 whereas matrine treatment could significantly reverse it, and the number of viruses of PRRSV also decreased. In this study, the method of network pharmacology was used to explore HSPA8 and HSP90AB1 which were the potential targets of matrine against PRRSV on Marc-145 cells.
猪繁殖与呼吸综合征（PRRS）是一种在全球范围内流行的动物传染病。在我们之前的研究中，有人提出苦参碱可以在体内和体外抑制PRRSV感染，但其抗病毒机制仍未确定。网络药理学可以很好地解决中药作用靶点研究中“多靶点、多途径”的难题。网络药理学结果表明，苦参碱通过靶向HSPA8和HSP90AB1发挥其抗PRRSV作用。实时荧光定量PCR和蛋白质印迹结果显示，PRRSV感染诱导HSPA8和HSP90AB1表达显著增加，而苦参碱处理可显著逆转这种增加，并且PRRSV病毒数量也减少。在本研究中，采用网络药理学方法在Marc-145细胞上探索苦参碱抗PRRSV的潜在靶点HSPA8和HSP90AB1。
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