Springer D L, Hackett P L, Miller R A, Buschbom R L
J Appl Toxicol. 1986 Apr;6(2):129-33. doi: 10.1002/jat.2550060212.
Previous studies performed in this laboratory indicated that exposure of rat fetuses to high-boiling coal liquids from 12-14 days of gestation (dg) induced a number of major malformations, including cleft palate, diaphragmatic hernia and small lungs. The study reported here was designed to determine postnatal viability and development of survivors following in utero exposure to Harmarville process solvent (HPS), a wide-boiling-range (150 to greater than 455 degrees C) coal liquid. For this study, 0.74 g kg-1 of the coal liquid was administered (by intragastric intubation) to rats from 12 to 14 dg. Offspring were evaluated for postnatal survival, growth and lung and thymus weights. Randomly selected pups from control and treated litters were killed and necropsied at 1, 3, 7 and 21 days postpartum. In addition, data for control pups were obtained at 0.25 and 0.5 days postpartum for comparison with body, lung and thymus weights of pups that died during this interval. Fifty-four per cent of the exposed pups and 9% of the control pups died between birth and 3 days postpartum. Of the treated pups that died, 10% (6/5; pups/litters) had cleft palate, 27% (17/9) had small lungs and 33% (21/8) had both cleft palate and small lungs. No gross malformations were observed in the remaining 30% of the dead pups. Microscopic examination of lungs from HPS-treated pups revealed no evident histological abnormalities. Body, lung and thymus weights for treated animals that died were significantly less than those of controls.(ABSTRACT TRUNCATED AT 250 WORDS)
此前在本实验室进行的研究表明，在妊娠12至14天（dg）时将大鼠胎儿暴露于高沸点煤液中会诱发一些主要畸形，包括腭裂、膈疝和肺发育不全。本文报告的研究旨在确定子宫内暴露于哈马维尔工艺溶剂（HPS，一种宽沸点范围（150至大于455摄氏度）的煤液）后存活者的产后活力和发育情况。在本研究中，从妊娠12至14天起，通过灌胃给大鼠施用0.74 g kg-1的煤液。对后代的产后存活、生长以及肺和胸腺重量进行评估。从对照和处理组的窝中随机挑选幼崽，在产后1、3、7和21天处死并进行尸检。此外，还获取了对照幼崽在产后0.25和0.5天的数据，以便与在此期间死亡的幼崽的体重、肺重和胸腺重进行比较。54%的暴露幼崽和9%的对照幼崽在出生至产后3天之间死亡。在死亡的处理组幼崽中，10%（6/5；幼崽/窝）有腭裂，27%（17/9）有肺发育不全，33%（21/8）既有腭裂又有肺发育不全。在其余30%的死亡幼崽中未观察到明显的严重畸形。对HPS处理组幼崽的肺进行显微镜检查未发现明显的组织学异常。死亡的处理组动物的体重、肺重和胸腺重显著低于对照组。（摘要截断于250字）
