Department of Food Nutrition and Health, School of Medicine and Health, Harbin Institute of Technology, Harbin 150001, China.
Institute of Microbiology, Heilongjiang Academy of Sciences, Harbin 150010, China.
Int J Mol Sci. 2021 Dec 15;22(24):13452. doi: 10.3390/ijms222413452.
has a strong carbohydrate utilization ability. This characteristic plays an important role in its gastrointestinal tract colonization and probiotic effects. LP-F1 presents a high carbohydrate utilization capacity. The genome analysis of 165 strains indicated the species has a plenty of carbohydrate metabolism genes, presenting a strain specificity. Furthermore, two-component systems (TCSs) analysis revealed that the species has more TCSs than other lactic acid bacteria, and the distribution of TCS also shows the strain specificity. In order to clarify the sugar metabolism mechanism under different carbohydrate fermentation conditions, the expressions of 27 carbohydrate metabolism genes, catabolite control protein A (CcpA) gene and TCSs genes were analyzed by quantitative real-time PCR technology. The correlation analysis between the expressions of regulatory genes and sugar metabolism genes showed that some regulatory genes were correlated with most of the sugar metabolism genes, suggesting that some TCSs might be involved in the regulation of sugar metabolism.
它具有很强的碳水化合物利用能力。这一特性在其胃肠道定植和益生菌作用中起着重要作用。LP-F1 具有很强的碳水化合物利用能力。对 165 株菌的基因组分析表明，该物种有大量的碳水化合物代谢基因，具有菌株特异性。此外，双组分系统（TCS）分析表明，该物种比其他乳酸菌具有更多的 TCS，并且 TCS 的分布也表现出菌株特异性。为了阐明不同碳水化合物发酵条件下的糖代谢机制，通过定量实时 PCR 技术分析了 27 个碳水化合物代谢基因、分解代谢物控制蛋白 A（CcpA）基因和 TCSs 基因的表达。调控基因与糖代谢基因表达的相关性分析表明，一些调控基因与大多数糖代谢基因相关，表明一些 TCS 可能参与糖代谢的调控。
