Department of Periodontology, Institute of Dentistry, University of Turku, Lemminkäisenkatu 2, 20520, Turku, Finland.
Department of Periodontology, Faculty of Dentistry, Istanbul University, Istanbul, Turkey.
Clin Oral Investig. 2023 May;27(5):2065-2074. doi: 10.1007/s00784-023-05010-5. Epub 2023 Apr 3.
The purposes of this study were to localize monocyte chemoattractant protein-1-induced protein-1 (MCPIP-1) and its suppressor mucosa-associated lymphoid tissue lymphoma translocation protein 1 (MALT-1) in gingival tissues and to profile their protein expression levels in relation to the clinical inflammation, Porphyromonas gingivalis colonization, and interleukin (IL)-8 levels.
Study samples were collected from two independent study populations: (1) Gingival tissues were collected from eight periodontally healthy individuals and eight periodontitis patients to localize MCPIP-1 and MALT-1 immunohistochemically, and (2) forty-one gingival tissue samples with marginal, mild, or moderate to severe inflammation were collected from 20 periodontitis patients to determine MCPIP-1 and MALT-1 levels using immunoblots, P. gingivalis levels with qPCR, P. gingivalis gingipain activities with fluorogenic substrates, and IL-8 levels with multiplex technique.
MCPIP-1 was detectable in the epithelium and in connective tissue, being especially prominent around the blood vessel walls in healthy periodontal tissues. MALT-1 was observed at all layers of gingival epithelium and especially around the accumulated inflammatory cells in connective tissue. No difference in gingival tissue MCPIP-1 and MALT-1 levels was observed in relation to the severity of gingival inflammation. MALT-1 levels were elevated (p = 0.023) with the increase in tissue P. gingivalis levels, and there was an association between MALT-1 and IL-8 levels (β = 0.054, p = 0.001).
Interactions of MALT-1 levels with gingival tissue P. gingivalis counts and IL-8 levels suggest that activation of MALT-1 can take part in P. gingivalis-regulated host immune responses.
Pharmacological targeting the crosstalk between immune response and MCPIP-1/MALT-1 may have benefits in periodontal treatment.
本研究旨在定位单核细胞趋化蛋白-1 诱导蛋白-1（MCPIP-1）及其抑制剂黏膜相关淋巴组织淋巴瘤易位蛋白 1（MALT-1）在牙龈组织中的位置，并分析其蛋白表达水平与临床炎症、牙龈卟啉单胞菌定植和白细胞介素（IL）-8 水平的关系。
研究样本取自两个独立的研究人群：（1）从 8 名牙周健康个体和 8 名牙周炎患者中收集牙龈组织，通过免疫组织化学定位 MCPIP-1 和 MALT-1；（2）从 20 名牙周炎患者中收集 41 份边缘性、轻度或中重度炎症的牙龈组织样本，通过免疫印迹法测定 MCPIP-1 和 MALT-1 水平、qPCR 测定牙龈卟啉单胞菌水平、荧光底物测定牙龈卟啉单胞菌牙龈蛋白酶活性、多指标技术测定 IL-8 水平。
MCPIP-1 在牙周组织健康时可在上皮组织和结缔组织中检测到，在血管壁周围尤为明显。MALT-1 在上皮组织的所有层中均可见，尤其是在结缔组织中积累的炎症细胞周围。牙龈组织中 MCPIP-1 和 MALT-1 水平与牙龈炎症的严重程度无关。MALT-1 水平随组织中牙龈卟啉单胞菌水平的升高而升高（p = 0.023），且 MALT-1 与 IL-8 水平之间存在关联（β=0.054，p=0.001）。
MALT-1 水平与牙龈组织中牙龈卟啉单胞菌计数和 IL-8 水平的相互作用表明，MALT-1 的激活可能参与牙龈卟啉单胞菌调节的宿主免疫反应。
针对免疫反应与 MCPIP-1/MALT-1 之间的相互作用进行药物靶向治疗可能有益于牙周治疗。
