School of of Life Sciences and Technology, Wuhan Polytechnic University, Wuhan, 430023, People's Republic of China.
Curr Microbiol. 2023 Apr 4;80(5):163. doi: 10.1007/s00284-023-03204-4.
Cottonseed meal is an important source of plant protein for the meal fodder materials. But its usage in animal breeding industry is limited by a type of toxic phenol, gossypol, that has toxic effects on animal health. Microbial degradation is a promising way to lower down gossypol in cottonseed meal. However, the molecular mechanisms of bio-degradation of gossypol is still unclear. In this study we isolated a gossypol-degrading bacterial strain, YL01, and sequenced its complete genome via Oxford Nanopore sequencing method. There is a chromosome (5,737,005 bp) and a plasmid (136,446 bp) in YL01. 5489 protein coding genes in total were functionally annotated. 16S rRNA analysis showed that YL01 taxonomically belongs to the genus of Raoultella. YL01 is the first published complete genome sequence of microbes capable of gossypol degradation. Gene function annotation showed that 126 protein coding genes may involve in gossypol catabolism. Sequence similarity analysis showed that, as the only gossypol-degrading strain in the genus of Raoultella, YL01 uniquely holds 260 genes that are not possessed by other Raoultella strains. Our work gives a preliminary list for genes responsible for gossypol degradation but further investigations are needed to completely disclose this molecular processes.
棉籽粕是饲料原料中植物蛋白的重要来源。但由于其中的棉酚是一种有毒的酚类物质，对动物健康有毒害作用，因此其在动物养殖行业的应用受到限制。微生物降解是降低棉籽粕中棉酚含量的一种很有前途的方法。然而，棉酚生物降解的分子机制仍不清楚。在本研究中，我们分离到了一株能够降解棉酚的细菌菌株 YL01，并通过 Oxford Nanopore 测序方法对其全基因组进行了测序。YL01 含有一条染色体（5737005bp）和一条质粒（136446bp），共注释了 5489 个蛋白编码基因。16S rRNA 分析表明，YL01 属于罗尔斯顿菌属。YL01 是第一个被报道的能够降解棉酚的微生物的完整基因组序列。基因功能注释表明，126 个蛋白编码基因可能参与棉酚代谢。序列相似性分析表明，作为罗尔斯顿菌属中唯一能够降解棉酚的菌株，YL01 独特地拥有 260 个其他罗尔斯顿菌属菌株所不具有的基因。我们的工作初步列出了与棉酚降解相关的基因，但需要进一步的研究来完全揭示这一分子过程。
