Lindena J, Küpper W, Trautschold I
J Clin Chem Clin Biochem. 1986 Jan;24(1):19-33. doi: 10.1515/cclm.1986.24.1.19.
In the mixed body lymph of the thoracic duct and in the defined organ lymph of the liver and the intestine, the catalytic activity concentrations of up to sixteen enzymes and the concentrations of albumin and protein were determined, as well as the transport rate of these substances and their lymph/plasma ratio. Thoracic duct lymph specimens were obtained from an extracorporeal lymph shunt in anaesthetized and conscious dogs and from short-term fistulas in anaesthetized rabbits, rats and mice. Additionally, rabbits and rats underwent passive motion of the hind limbs in another experimental trial. Thoracic duct flow in anaesthetized dogs is only half that seen in conscious dogs, due to bypassed muscular lymph. A similar flow change is seen during passive motion of hind limbs in anaesthetized rabbits and rats. From a literature review of flow in the four main lymphatics of the body, it is concluded that the thoracic duct flow should account for 50-70% of total body lymph flow. In the anaesthetized state, flow is mainly of visceral origin. In the conscious state and during passive motion the increased flow is of muscular origin. In the latter case, the catalytic activities of enzymes like lactate dehydrogenase, malate dehydrogenase, creatine kinase, aldolase and phosphohexose isomerase, increase in lymph as does their lymph/plasma ratio. These enzymes have high catalytic activities in muscle. Their transport into the blood increases 2-3-fold, due to a doubling of lymph flow. Reported data for anaesthetized and immobile animals therefore far underestimate the significance of thoracic duct enzyme transport. Liver lymph was obtained from anaesthetized dogs and rabbits. Our finding that lymph catalytic activity for several enzymes is higher than in plasma is not compatible with the proposed delivery of plasma proteins directly into the sinusoidal space without prior mixing with the Space of Disse. Enzymes in liver lymph should derive from parenchymal and endothelial lining cells. Their site of delivery from the hepatocyte seems different from that of proteins. Liver lymph is an important transport route of enzymes into the blood. Intestinal lymph was sampled from anaesthetized dogs, rabbits and rats. It was shown that most enzymes from the intestine are primarily released into the interstitial space and from there are transported via the lymph into the blood.
在胸导管的混合体淋巴以及肝脏和肠道的特定器官淋巴中，测定了多达16种酶的催化活性浓度、白蛋白和蛋白质的浓度，以及这些物质的转运速率及其淋巴/血浆比值。胸导管淋巴样本取自麻醉和清醒犬的体外淋巴分流，以及麻醉兔、大鼠和小鼠的短期瘘管。此外，在另一项实验中，对兔和大鼠的后肢进行了被动运动。麻醉犬的胸导管流量仅为清醒犬的一半，这是由于肌肉淋巴被绕过。在麻醉兔和大鼠的后肢被动运动期间也观察到类似的流量变化。通过对身体四条主要淋巴管流量的文献综述得出结论，胸导管流量应占全身淋巴流量的50 - 70%。在麻醉状态下，流量主要来源于内脏。在清醒状态和被动运动期间，增加的流量来源于肌肉。在后一种情况下，乳酸脱氢酶、苹果酸脱氢酶、肌酸激酶、醛缩酶和磷酸己糖异构酶等酶在淋巴中的催化活性及其淋巴/血浆比值都会增加。这些酶在肌肉中具有高催化活性。由于淋巴流量加倍，它们进入血液的转运增加2 - 3倍。因此，报道的关于麻醉和不动动物的数据远远低估了胸导管酶转运的重要性。肝脏淋巴取自麻醉犬和兔。我们发现几种酶在淋巴中的催化活性高于血浆，这与所提出的血浆蛋白直接输送到肝血窦空间而无需事先与狄氏间隙混合的观点不一致。肝脏淋巴中的酶应来源于实质细胞和内皮衬里细胞。它们从肝细胞的输送部位似乎与蛋白质不同。肝脏淋巴是酶进入血液的重要运输途径。肠道淋巴取自麻醉犬、兔和大鼠。结果表明，肠道中的大多数酶主要释放到间质空间，然后从那里通过淋巴输送到血液中。
