Lindena J, Küpper W, Trautschold I
J Clin Chem Clin Biochem. 1982 Feb;20(2):95-102. doi: 10.1515/cclm.1982.20.2.95.
The effect of hypoxia lasting one hour on the hind leg muscle of anaesthetised dogs was investigated. Ten enzyme activities in plasma and leg lymph, and the lymphatic transport of these enzymes were investigated. Enzymes with high activity in muscle, like creatine kinase, lactate dehydrogenase, malate dehydrogenase and adenylate kinase only show an increase in the plasma, if lymph--propulsed by passive motion of the hind leg--can reach the intravascular space. This effect is independent of transient hypoxia. Depending on the level of enzyme activity in the muscle, the activity in leg lymph is up to 6-fold higher than in plasma. Enzymes from muscle have to be transported into the blood by lymph flow and not via a direct interstitial-venous entry. The results are discussed especially with respect to enzyme activity changes in plasma during physical exercise.
研究了持续一小时的缺氧对麻醉犬后腿肌肉的影响。检测了血浆和腿部淋巴液中的十种酶活性，并研究了这些酶的淋巴转运情况。肌肉中活性较高的酶，如肌酸激酶、乳酸脱氢酶、苹果酸脱氢酶和腺苷酸激酶，只有在通过后腿被动运动推动淋巴液进入血管空间时，血浆中的活性才会增加。这种效应与短暂缺氧无关。根据肌肉中酶活性的水平，腿部淋巴液中的活性比血浆中的活性高6倍。肌肉中的酶必须通过淋巴液流入血液，而不是通过直接的间质-静脉进入途径。尤其结合体育锻炼期间血浆中酶活性的变化对这些结果进行了讨论。
