Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, Heinrich-Buff-Ring 26-32, 35392, Giessen, Germany.
Institut für Ernährungs- und Lebensmittelwissenschaften, Lebensmittelmikrobiologie und -hygiene, Rheinische Friedrich-Wilhelms-Universität Bonn, Bonn, Germany.
Antonie Van Leeuwenhoek. 2023 Sep;116(9):883-892. doi: 10.1007/s10482-023-01852-x. Epub 2023 Jun 20.
A Gram-strain positive, aerobic, endospore-forming bacterial strain (JJ-246) was isolated from the rhizosphere of Zea mays. The 16S rRNA gene sequence similarity comparisons showed a most closely relationship to Paenibacillus oenotherae DT7-4 (98.4%) and Paenibacillus xanthinolyticus 11N27 (98.0%). The pairwise average nucleotide identity and digital DNA-DNA hybridisation values of the JJ-246 genome assembly against publicly available Paenibacillus type strain genomes were below 82% and 33%, respectively. The draft genome of JJ-246 shared many putative plant-beneficial functions contributing (PBFC) genes, related to plant root colonisation, oxidative stress protection, degradation of aromatic compounds, plant growth-promoting traits, disease resistance, drug and heavy metal resistance, and nutrient acquisition. The quinone system of strain JJ-246, the polar lipid profile and the major fatty acids were congruent with those reported for members of the genus Paenibacillus. JJ-246 was shown to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus plantiphilus sp. nov. is proposed, with JJ-246 (= LMG 32093 = CCM 9089 = CIP 111893) as the type strain.
一株革兰氏阳性、需氧、产芽孢的细菌菌株（JJ-246）从玉米根际中分离得到。16S rRNA 基因序列相似性比较表明，该菌株与奥奈达伯克霍尔德氏菌 DT7-4（98.4%）和黄粘质沙雷氏菌 11N27（98.0%）最为密切相关。JJ-246 基因组组装与公开的伯克霍尔德氏菌模式菌株基因组的成对平均核苷酸同一性和数字 DNA-DNA 杂交值分别低于 82%和 33%。JJ-246 的草图基因组共享了许多与植物有益功能相关的（PBFC）基因，这些基因与植物根定殖、氧化应激保护、芳香族化合物降解、植物生长促进特性、抗病性、药物和重金属抗性以及养分获取有关。菌株 JJ-246 的醌系统、极性脂谱和主要脂肪酸与伯克霍尔德氏菌属的报道一致。JJ-246 被证明代表了伯克霍尔德氏菌属的一个新种，现提议将其命名为植物生孢伯克霍尔德氏菌（Paenibacillus plantiphilus）sp. nov.，其模式菌株为 JJ-246（= LMG 32093 = CCM 9089 = CIP 111893）。
