Department of Animal and Grassland Sciences, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan.
Center for Animal Disease Control, University of Miyazaki, Miyazaki, Japan.
PLoS Negl Trop Dis. 2023 Apr 4;17(4):e0011259. doi: 10.1371/journal.pntd.0011259. eCollection 2023 Apr.
Diarrheagenic Escherichia coli (DEC) is a group of bacterial pathogens that causes life-threatening diarrhea in children in developing countries. However, there is limited information on the characteristics of DEC isolated from patients in these countries. A detailed genomic analysis of 61 DEC-like isolates from infants with diarrhea was performed to clarify and share the characteristics of DEC prevalent in Vietnam.
DEC was classified into 57 strains, including 33 enteroaggregative E. coli (EAEC) (54.1%), 20 enteropathogenic E. coli (EPEC) (32.8%), two enteroinvasive E. coli (EIEC) (3.3%), one enterotoxigenic E. coli (ETEC), and one ETEC/EIEC hybrid (1.6% each), and surprisingly into four Escherichia albertii strains (6.6%). Furthermore, several epidemic DEC clones showed an uncommon combination of pathotypes and serotypes, such as EAEC Og130:Hg27, EAEC OgGp9:Hg18, EAEC OgX13:H27, EPEC OgGp7:Hg16, and E. albertii EAOg1:HgUT. Genomic analysis also revealed the presence of various genes and mutations associated with antibiotic resistance in many isolates. Strains that demonstrate potential resistance to ciprofloxacin and ceftriaxone, drugs recommended for treating childhood diarrhea, accounted for 65.6% and 41%, respectively.
Our finding indicate that the routine use of these antibiotics has selected resistant DECs, resulting in a situation where these drugs do not provide in therapeutic effects for some patients. Bridging this gap requires continuous investigations and information sharing regarding the type and distribution of endemic DEC and E. albertii and their antibiotic resistance in different countries.
肠致病性大肠杆菌（DEC）是一组细菌病原体，可导致发展中国家儿童发生危及生命的腹泻。然而，关于这些国家患者分离的 DEC 的特征，信息有限。对 61 例腹泻婴儿的 DEC 样分离株进行了详细的基因组分析，以阐明和共享越南流行的 DEC 特征。
DEC 分为 57 株，包括 33 株聚集性肠致病性大肠杆菌（EAEC）（54.1%）、20 株肠致病性大肠杆菌（EPEC）（32.8%）、2 株肠侵袭性大肠杆菌（EIEC）（3.3%）、1 株肠毒性大肠杆菌和 1 株 ETEC/EIEC 杂交株（各占 1.6%），令人惊讶的是还有 4 株 Escherichia albertii 菌株（6.6%）。此外，一些流行的 DEC 克隆显示出不常见的血清型和血清型组合，如 EAEC Og130:Hg27、EAEC OgGp9:Hg18、EAEC OgX13:H27、EPEC OgGp7:Hg16 和 E. albertii EAOg1:HgUT。基因组分析还显示，许多分离株存在与抗生素耐药性相关的各种基因和突变。对环丙沙星和头孢曲松具有潜在耐药性的菌株分别占 65.6%和 41%，这两种药物均推荐用于治疗儿童腹泻。
我们的研究结果表明，这些抗生素的常规使用已经选择了耐药的 DEC，导致一些患者无法从这些药物中获得治疗效果。弥合这一差距需要不断调查和信息共享，了解不同国家中地方性 DEC 和 Escherichia albertii 的类型和分布及其抗生素耐药性。
