Obstetrics and Gynaecology Department, Faculty of Medicine, Minia University, Minia, Egypt.
Dairy Department, Faculty of Agriculture, Minia University, Minia, Egypt.
Pharm Res. 2023 Oct;40(10):2281-2290. doi: 10.1007/s11095-023-03505-0. Epub 2023 Apr 4.
Ovarian cancer is the most lethal cancer in gynaecology. Surgery, chemotherapy, and radiotherapy are the most often used cancer-fighting strategies. Post-surgery infection is fairly prevalent, especially among people with insufficient immunity. Zinc oxide nanoparticles (ZnOnps) have amazing biomedical features as anticancer and antibacterial agents.
We investigated the behaviour of ZnOnps synthesized by green methods on ovarian cancers using established human ovarian cancer cell lines, besides the antibacterial action toward models of gram + ve and gram -ve bacteria. The cytotoxic effect of ZnOnps was calculated using a Sulforhodamine B (SRB) trial. Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) were tested as models for gram + ve and gram -ve bacteria. The selected bacteria were subjected to concentrations of 20, 40, 80, and 100 μg/ml.
The synthesized ZnOnps induced 50% inhibitory concentration (IC50) at a concentration of 27.45 μg/ml. The diameter of inhibition ranged between 20.16 ± 0.16 and 27 ± 0.57 mm for S. aureus and 25.66 ± 0.33 to 31 ± 0.33 mm for E. coli. ZnOnps antagonistic effect statistically differed with neomycin, cefaclor, and cefadroxil.
Green synthesis of ZnOnps is easily prepared, low cost, non-toxic, and eco-friendly. Their cytotoxic action on SKOV3 cells and their antibacterial characteristics pave the way to be an alternative therapy for ovarian cancer and S. aureus and E. coli infection.
卵巢癌是妇科最致命的癌症。手术、化疗和放疗是最常用的抗癌策略。手术后感染相当普遍，尤其是免疫力低下的人群。氧化锌纳米粒子（ZnOnps）作为抗癌和抗菌剂具有惊人的生物医学特性。
我们使用已建立的人卵巢癌细胞系研究了绿色方法合成的 ZnOnps 对卵巢癌的作用，以及对革兰氏阳性和革兰氏阴性细菌模型的抗菌作用。使用 Sulforhodamine B（SRB）试验计算 ZnOnps 的细胞毒性作用。金黄色葡萄球菌（S. aureus）和大肠杆菌（E. coli）被用作革兰氏阳性和革兰氏阴性细菌的模型。选择的细菌浓度分别为 20、40、80 和 100μg/ml。
合成的 ZnOnps 在浓度为 27.45μg/ml 时诱导 50%抑制浓度（IC50）。抑制直径在 20.16±0.16 和 27±0.57mm 之间，用于 S. aureus，在 25.66±0.33 和 31±0.33mm 之间，用于 E. coli。ZnOnps 的拮抗作用与新霉素、头孢克洛和头孢羟氨苄有统计学差异。
ZnOnps 的绿色合成制备简便、成本低、无毒、环保。它们对 SKOV3 细胞的细胞毒性作用及其抗菌特性为卵巢癌和金黄色葡萄球菌和大肠杆菌感染的替代疗法铺平了道路。
