Moawad Raghda, Abdallah Yasmine, Mohany Mohamed, Al-Rejaie Salim S, Djurasevic Sinisa, Ramadan Mohamed Fawzy, Mousa Ahmed Bakr
Dairy Department, Faculty of Agriculture, Minia University, Minia, 61519, Egypt.
Plant Pathology Department, Faculty of Agriculture, Minia University, Minia, 61519, Egypt.
Sci Rep. 2025 Jan 3;15(1):727. doi: 10.1038/s41598-024-82679-y.
Nanomedical applications have increased significantly. This work aimed to fabricate and characterize cobalt oxide nanoparticles (CoOnps) synthesized biologically via aqueous Alhagi maurorum extract and evaluate their cytotoxic and antimicrobial impacts. Green-synthesized CoOnps were prepared and analyzed using UV-Vis spectrophotometer UV-vis, Scanning electron microscopy (SEM), Transmission electron microscopy TEM, Energy dispersive X-ray analysis EDAX, Fourier transform infrared, FTIR, and X-ray diffraction (XRD). In vitro traits of green-synthesized CoOnps were studied on ovarian cancer cells (SKOV3) using a Sulforhodamine B (SRB) method. The cytotoxic effect and IC50 were estimated. Moreover, concentrations of 10, 30, 40, 70, 100, 200, 300, 400 and 500 μg/mL CoOnps were applied to investigate their antimicrobial effect against Listeria, Staphylococcus aureus and Streptococcus as gram +ve pathogenic bacteria, Bifidobacterium bifidum 2203, Bifidobacterium bifidum LMG 10,645, Bifidobacterium breve LMC 017, Bifidobacterium angulatum 2238 and Bifidobacterium longum ATCC 15,707 as probiotics, E. coli as gram -ve bacterial model and yeast strain Candida albicans. CoOnps showed anti-ovarian cancer effects at 24.02 μg/mL. Furthermore, it exerted antimicrobial activity versus Listeria, Streptococcus, S. aureus, and E. coli were 31.66 ± 0.88, 24.33 ± 2.08, 25.66 ± 0.33, and 33.00 ± 6.08; however, they did not suppress the growth of Candida albicans and all tested Bifidobacterial strains up to concentrations of 500 μg/mL with significant difference compared to all concentrations p < 0.05. Green synthesis of CoOnps is a low-cost, eco-friendly and easily prepared method. Its impressive features as cytotoxic SKOV3, a cell line ovarian cancer and antibacterial effect for some gram +ve and -ve bacteria, besides maintaining probiotics, could candidate them as competitive agents for medical, pharmacological, agricultural and food applications.
纳米医学应用显著增加。本研究旨在制备并表征通过黑果枸杞水提取物生物合成的氧化钴纳米颗粒（CoOnps），并评估其细胞毒性和抗菌作用。使用紫外可见分光光度计（UV-vis）、扫描电子显微镜（SEM）、透射电子显微镜（TEM）、能量色散X射线分析（EDAX）、傅里叶变换红外光谱（FTIR）和X射线衍射（XRD）对绿色合成的CoOnps进行制备和分析。采用磺酰罗丹明B（SRB）法研究绿色合成的CoOnps对卵巢癌细胞（SKOV3）的体外特性。估算细胞毒性作用和半数抑制浓度（IC50）。此外，应用浓度为10、30、40、70、100、200、300、400和500μg/mL的CoOnps研究其对革兰氏阳性病原菌李斯特菌、金黄色葡萄球菌和链球菌，益生菌双歧双歧杆菌2203、双歧双歧杆菌LMG 10645、短双歧杆菌LMC 017、角双歧杆菌2238和长双歧杆菌ATCC 15707，革兰氏阴性菌模型大肠杆菌以及酵母菌株白色念珠菌的抗菌效果。CoOnps在浓度为24.02μg/mL时显示出抗卵巢癌作用。此外，它对李斯特菌、链球菌、金黄色葡萄球菌和大肠杆菌的抗菌活性分别为31.66±0.88、24.33±2.08、25.66±0.33和33.00±6.08；然而，在浓度高达500μg/mL时，它们并未抑制白色念珠菌和所有测试双歧杆菌菌株的生长，与所有浓度相比差异显著（p<0.05）。绿色合成CoOnps是一种低成本、环保且易于制备的方法。其对卵巢癌细胞系SKOV3具有细胞毒性以及对一些革兰氏阳性和阴性细菌具有抗菌作用，同时还能维持益生菌活性，这些令人印象深刻的特性使其有望成为医学、药理学、农业和食品应用中的有竞争力的制剂。
