Hussein Muthanna O, Abdulhameed Ahmed Saud
Department of Clinical Laboratory Sciences, College of Pharmacy, University of Anbar, Ramadi, Iraq.
Department of Pharmaceutical Chemistry, College of Pharmacy, University of Anbar, Ramadi, Iraq.
Appl Biochem Biotechnol. 2025 Feb;197(2):1073-1089. doi: 10.1007/s12010-024-05066-3. Epub 2024 Oct 1.
In the current research, a chitosan/broccoli extract/ZnO nanoparticle (CH/BE/ZnO) bionanocomposite was created. The physicochemical properties of CH/BE/ZnO bionanocomposite were investigated using a variety of methods, including field emission scanning electron microscopy (FESEM), elemental analysis (CHN-O), X-ray diffraction (XRD), Fourier transform infrared spectrum (FTIR), Brunauer-Emmett-Teller (BET), and transmission electron microscopy (TEM). The CH/BE/ZnO bionanocomposite's biological activity was assessed by examining its cytotoxicity capabilities against a bone cancer cell line (MG63). The total pore volume and specific surface area of CH/BE/ZnO are 0.134 cm/g and 16.99 m/g, respectively. The IC50 results for CH/BE/ZnO bionanocomposite in bone cancer investigations using the MTT test against the MG63 cell line was 115 μg/mL. The results indicate that the CH/BE/ZnO bionanocomposite is an effective chemotherapeutic agent against human osteosarcoma. The CH/BE/ZnO bionanocomposite showed high performance and structure, which means innovating nanomaterial agents for biological applications in the future.
在当前的研究中，制备了一种壳聚糖/西兰花提取物/氧化锌纳米颗粒（CH/BE/ZnO）生物纳米复合材料。采用多种方法对CH/BE/ZnO生物纳米复合材料的物理化学性质进行了研究，包括场发射扫描电子显微镜（FESEM）、元素分析（CHN-O）、X射线衍射（XRD）、傅里叶变换红外光谱（FTIR）、布鲁诺尔-埃米特-泰勒（BET）和透射电子显微镜（TEM）。通过检测CH/BE/ZnO生物纳米复合材料对骨癌细胞系（MG63）的细胞毒性能力，评估其生物活性。CH/BE/ZnO的总孔体积和比表面积分别为0.134 cm/g和16.99 m/g。在针对MG63细胞系的骨癌研究中，使用MTT试验得出CH/BE/ZnO生物纳米复合材料的IC50结果为115μg/mL。结果表明，CH/BE/ZnO生物纳米复合材料是一种有效的抗人骨肉瘤化疗药物。CH/BE/ZnO生物纳米复合材料表现出高性能和结构，这意味着未来将为生物应用创新纳米材料制剂。
