Jensen J A, Hunt T K, Scheuenstuhl H, Banda M J
Lab Invest. 1986 May;54(5):574-8.
Macrophages secrete substances that stimulate angiogenesis and mitogenesis at wound sites. To test whether metabolic characteristics of the wound such as high lactate concentration and low pH may regulate the expression of these substances, macrophages were cultured in lactalbumin hydrolysate and then in increasing concentrations of lactate, pyruvate, and hydrogen ions, and cell-free supernatants were collected. Cultures incubated in concentrations of lactate approximating those observed in wounds secreted an angiogenesis factor or factors, but cultures incubated in equivalent concentrations of pyruvate or at pH 6.2 did not. These observations seem to be specific to macrophages because capillary endothelial cells and fibroblasts failed to secrete an angiogenesis factor when cultured under hypoxic conditions or in high concentrations of lactate. None of these conditions changed the expression of macrophage mitogens.
巨噬细胞分泌能刺激伤口部位血管生成和有丝分裂的物质。为了测试伤口的代谢特征（如高乳酸浓度和低pH值）是否可能调节这些物质的表达，将巨噬细胞先在乳白蛋白水解物中培养，然后在乳酸、丙酮酸和氢离子浓度逐渐增加的环境中培养，并收集无细胞上清液。在接近伤口中观察到的乳酸浓度下培养的细胞分泌了一种或多种血管生成因子，但在等浓度丙酮酸或pH值为6.2的环境中培养的细胞则没有。这些观察结果似乎是巨噬细胞特有的，因为毛细血管内皮细胞和成纤维细胞在低氧条件下或高浓度乳酸中培养时未能分泌血管生成因子。这些条件均未改变巨噬细胞有丝分裂原的表达。
