Wuhan Academy of Agricultural Sciences, Wuhan 430072, China.
Hubei Key Laboratory of Environmental and Health Effects of Persistent Toxic Substances, School of Environment and Health, Jianghan University, Wuhan 430056, China.
Anal Chem. 2023 Apr 18;95(15):6417-6424. doi: 10.1021/acs.analchem.3c00251. Epub 2023 Apr 9.
Rapid and sensitive detection of foodborne bacteria is of great significance in guaranteeing food safety and preventing foodborne diseases. A bifunctional Au@Pt core-shell nanozyme with excellent catalytic properties and high surface-enhanced Raman scattering (SERS) activity was developed for the highly sensitive detection of based on a label-free SERS strategy. The ultrathin Pt shell (about 1 nm) can catalyze Raman-inactive molecules into Raman-active reporters, greatly amplifying the amount of signal molecules. Moreover, the Au core serves as an active SERS substrate to enhance the signal of reporter molecules, further significantly improving the detection sensitivity. Benefiting from the excellent properties, such a bifunctional Au@Pt nanozyme was integrated with a magnetic immunoassay to construct a label-free SERS platform for the highly sensitive detection of with a low detection limit of 10 CFU mL. Also, the Au@Pt-based SERS platform exhibited excellent selectivity and was successfully utilized for the detection of in milk samples by a portable Raman spectrometer. Our demonstration of the bifunctional nanozyme-based SERS strategy provides an efficient pathway to improve the sensitivity of label-free SERS detection of pathogens and holds great promise in food safety, environmental analysis, and other biosensing fields.
快速、灵敏地检测食源性细菌对于保障食品安全和预防食源性疾病至关重要。本研究开发了一种具有优异催化性能和高表面增强拉曼散射（SERS）活性的双功能 Au@Pt 核壳纳米酶，基于无标记 SERS 策略，实现了对的高灵敏度检测。超薄的 Pt 壳（约 1nm）可以将拉曼非活性分子催化成拉曼活性报告分子，极大地放大了信号分子的数量。此外，Au 核作为活性 SERS 基底，增强了报告分子的信号，进一步显著提高了检测灵敏度。得益于这些优异的性能，这种双功能 Au@Pt 纳米酶被集成到磁性免疫测定中，构建了一种无标记 SERS 平台，用于对进行高灵敏度检测，检测限低至 10CFU mL。此外，基于 Au@Pt 的 SERS 平台表现出优异的选择性，并成功地用于便携式拉曼光谱仪对牛奶样品中的进行检测。我们对基于双功能纳米酶的 SERS 策略的验证，为提高无标记 SERS 检测病原体的灵敏度提供了一种有效的途径，在食品安全、环境分析和其他生物传感领域具有广阔的应用前景。
