Dong J X, Li T, Chao S, Gao Y, Zhou L X, Zhao X Y
Department of Clinical Laboratory, Beijing Tsinghua ChangGung Hospital, School of Clinical Medicine,Tsinghua University, Beijing 102218, China.
School of Laboratory Medicine, Dalian Medical University, Dalian 116000, China.
Zhonghua Yu Fang Yi Xue Za Zhi. 2023 Apr 6;57(4):566-571. doi: 10.3760/cma.j.cn112150-20220511-00471.
As a single-center retrospective study, we analyzed the results of rotavirus and human adenovirus antigens in stool samples with colloidal gold immunochromatography method in children with acute gastroenteritis under the age of five who were treated in our hospital from 2019 to 2022. After excluding nonconforming cases and duplicate cases, 2 896 cases were included, of which 559 cases were detected with at least one viral antigen. According to the test results, they were divided into RV positive group, HAdV positive group and RV & HAdV double positive group. The gender, age, seasonal distribution, clinical symptoms and related laboratory tests were compared and analyzed with test, analysis of variance and nonparametric test. Among the single samples from 2 896 children, the positive rate of RV antigen was 6.21% (180/2 896), the positive rate of HAdV antigen was 10.91% (316/2 896), and the double positive rate of RV & HAdV was 2.18% (63/2 896). The positive rate of HAdV antigen in 2021 was 16.11%, a significant increase compared with 6.20% in 2020. RV infection has obvious seasonality, and spring and winter are the seasons with high incidence of infection (=74.018, <0.001), while HAdV infection has no obvious seasonality (=2.110, =0.550), showing sporadic infection throughout the year. The proportions of fever and vomiting symptoms in children with RV infection were significantly higher than those in the HAdV infection group (=40.401, 0.001; =32.593, <0.001), but the positive rate of white blood cells in the stool was significantly lower than that in the HAdV infection group (=13.741,<0.01). In summary, paying attention to the epidemiological changes of RV and HAdV is of great significance for clinical diagnosis and treatment and disease prevention and control.
作为一项单中心回顾性研究，我们采用胶体金免疫层析法分析了2019年至2022年在我院接受治疗的5岁以下急性肠胃炎患儿粪便样本中轮状病毒和人腺病毒抗原的检测结果。排除不符合标准的病例和重复病例后，共纳入2896例，其中559例检测到至少一种病毒抗原。根据检测结果，将其分为轮状病毒（RV）阳性组、人腺病毒（HAdV）阳性组和RV与HAdV双阳性组。采用t检验、方差分析和非参数检验对性别、年龄、季节分布、临床症状及相关实验室检查结果进行比较分析。在2896例患儿的单样本中，RV抗原阳性率为6.21%（180/2896），HAdV抗原阳性率为10.91%（316/2896），RV与HAdV双阳性率为2.18%（63/2896）。2021年HAdV抗原阳性率为16.11%，较2020年的6.20%显著升高。RV感染具有明显的季节性，春季和冬季是感染高发季节（χ² =74.018，P<0.001），而HAdV感染无明显季节性（χ² =2.110，P =0.550），全年呈散发性感染。RV感染患儿发热和呕吐症状的比例显著高于HAdV感染组（t =40.401，P<0.001；t =32.593，P<0.001），但粪便白细胞阳性率显著低于HAdV感染组（t =13.741，P<0.01）。综上所述，关注RV和HAdV的流行病学变化对临床诊断治疗及疾病防控具有重要意义。
