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在酿酒酵母减数分裂和子囊孢子形成过程中使用分步酶作为标记物。

The use of step enzymes as markers during meiosis and ascospore formation in Saccharomyces cerevisiae.

作者信息

Matur A, Berry D R

出版信息

J Gen Microbiol. 1978 Dec;109(2):205-13. doi: 10.1099/00221287-109-2-205.

DOI:10.1099/00221287-109-2-205
PMID:370342
Abstract

The activities of ornithine aminotransferase, sucrase and acid and alkaline phosphatases have been studied throughout sporulation in Saccharomyces cerevisiae. The same enzymes were monitored during synchronous vegetative growth. Each of these enzymes has been demonstrated to increase in a 'step' manner during both growth and sporulation. Alkaline phosphatase increased in a two-step manner whereas the others increased in a single step. The times of increase of these enzymes formed a similar sequence during both sporulation and growth. It has been proposed that these enzymes are under a common mechanism of control during growth and sporulation and that the sequence of enzyme appearance may be used as markers of the sporulation process.

摘要

在酿酒酵母的整个孢子形成过程中,对鸟氨酸转氨酶、蔗糖酶以及酸性和碱性磷酸酶的活性进行了研究。在同步营养生长期间也对相同的酶进行了监测。已证明这些酶中的每一种在生长和孢子形成过程中均以“阶梯”方式增加。碱性磷酸酶以两步方式增加,而其他酶以单步方式增加。这些酶增加的时间在孢子形成和生长过程中形成了相似的序列。有人提出,这些酶在生长和孢子形成过程中处于共同的控制机制之下,并且酶出现的序列可用作孢子形成过程的标记。

相似文献

1
The use of step enzymes as markers during meiosis and ascospore formation in Saccharomyces cerevisiae.在酿酒酵母减数分裂和子囊孢子形成过程中使用分步酶作为标记物。
J Gen Microbiol. 1978 Dec;109(2):205-13. doi: 10.1099/00221287-109-2-205.
2
Developmental regulation of a sporulation-specific enzyme activity in Saccharomyces cerevisiae.酿酒酵母中一种孢子形成特异性酶活性的发育调控。
Mol Cell Biol. 1982 Feb;2(2):171-8. doi: 10.1128/mcb.2.2.171-178.1982.
3
Effect of ammonium ions on activity of hydrolytic enzymes during sporulation of yeast.铵离子对酵母孢子形成过程中水解酶活性的影响。
J Bacteriol. 1979 Jun;138(3):1022-5. doi: 10.1128/jb.138.3.1022-1025.1979.
4
Sporulation in D-glucosamine auxotrophs of Saccharomyces cerevisiae: meiosis with defective ascospore wall formation.酿酒酵母D-葡萄糖胺营养缺陷型中的孢子形成:减数分裂时子囊孢子壁形成缺陷。
J Bacteriol. 1975 Dec;124(3):1545-57. doi: 10.1128/jb.124.3.1545-1557.1975.
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Proteinase activities of Saccharomyces cerevisiae during sporulation.酿酒酵母在孢子形成过程中的蛋白酶活性。
J Bacteriol. 1975 Nov;124(2):863-9. doi: 10.1128/jb.124.2.863-869.1975.
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A particulate form of alkaline phosphatase in the yeast, Saccharomyces cerevisiae.酵母(酿酒酵母)中碱性磷酸酶的一种颗粒形式。
Biochim Biophys Acta. 1981 Feb 13;657(2):482-94. doi: 10.1016/0005-2744(81)90333-8.
7
Protein degradation and proteinases during yeast sporulation.酵母孢子形成过程中的蛋白质降解与蛋白酶
Eur J Biochem. 1976 Feb 2;62(1):65-76. doi: 10.1111/j.1432-1033.1976.tb10098.x.
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Aberrant nuclear behavior at meiosis and anucleate spore formation by sporulation-deficient (SPO) mutants of Saccharomyces cerevisiae.酿酒酵母孢子形成缺陷(SPO)突变体在减数分裂时的异常核行为及无核孢子形成。
Exp Cell Res. 1974 Jan;83(1):166-74. doi: 10.1016/0014-4827(74)90700-9.
9
[Sporulation of yeast cells, with special reference to the turnover of nucleic acids during sporulation (author's transl)].[酵母细胞的孢子形成,特别涉及孢子形成过程中核酸的周转(作者译)]
Tanpakushitsu Kakusan Koso. 1977;22(9):1112-22.
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[Comparative effects of fluoride on three enzymes, hydrolyzing pyrophosphate - acid and alkaline phosphatases and inorganic pyrophosphatase].[氟对三种酶(水解焦磷酸的酸性和碱性磷酸酶以及无机焦磷酸酶)的比较作用]
Biokhimiia. 1982 Aug;47(8):1289-92.

引用本文的文献

1
In vitro synthesis of repressible yeast acid phosphatase: identification of multiple mRNAs and products.可阻遏酵母酸性磷酸酶的体外合成:多种mRNA和产物的鉴定
Proc Natl Acad Sci U S A. 1980 Aug;77(8):4504-8. doi: 10.1073/pnas.77.8.4504.
2
Glucose repression and autolysis of Saccharomyces cerevisiae cells: alterations in the cytochemical localization of acid phosphatase.酿酒酵母细胞的葡萄糖阻遏与自溶:酸性磷酸酶细胞化学定位的改变
Histochem J. 1980 Jan;12(1):57-69. doi: 10.1007/BF01066537.
3
Physiological control of repressible acid phosphatase gene transcripts in Saccharomyces cerevisiae.
酿酒酵母中可阻遏酸性磷酸酶基因转录物的生理调控
Mol Cell Biol. 1983 May;3(5):839-53. doi: 10.1128/mcb.3.5.839-853.1983.