Halfaoui Zehor, Menoueri Nabil Mohamed, Bendali Lyes Mohamed
Veterinary Institute, University of Blida 1, Road of Soumaa, BP 270, Blida 09000, Algeria.
Medical Analysis Laboratory, Miliana, BP 44200, Algeria.
Vet World. 2017 Jul;10(7):830-835. doi: 10.14202/vetworld.2017.830-835. Epub 2017 Jul 28.
Colibacillosis is considered as one of the major bacterial infections in avian pathology. The excessive use of antibiotics reduced their effectiveness, which eventually led to the risk of emergence of antibiotic resistance. The aim of this study was to isolate, identify and serotype the pathogenic strains and to determine their antibiotic susceptibility.
A total of 180 samples from different organs of broilers with colibacillosis lesions were collected (liver, spleen, lung, and heart) in center of Algeria. The isolation and identification of were carried out using conventional techniques. Then, these strains were serotyped and tested over 13 antibiotics.
A total of 156 strains of were isolated. Serotyping results showed that 50 strains belong to 3 serotypes (23 for O1, 11 for O2, 16 for O78) which represent 32% of isolates. The antimicrobial susceptibility test, presented high level of resistance to tetracyclines (94.12%), flumequine (91.5%), sulfamethoxazole-trimethoprim (88.89%), enrofloxacin (86.27%), nalidixic acid (85.62%), ampicillin (83.01%) and doxycycline (75.81%), medium level resistance to chloramphenicol (39.22%), and amoxicillin-clavulanic acid (43.13%). All the strains were susceptible to cefotaxime, excepting three, which presented an extended spectrum β-lactamase (ESBL). In addition, the results of multi-resistance showed that all the strains were resistant at the minimum to two antibiotics and 66.66% of strains were resistant to at least seven antibiotics.
The antibiotic resistance continues to rise at an alarming rate, and the emergence of ESBL is considered as a threat for public health.
大肠杆菌病被认为是禽类病理学中的主要细菌感染之一。抗生素的过度使用降低了其有效性，最终导致出现抗生素耐药性的风险。本研究的目的是分离、鉴定致病性菌株并进行血清分型，以及确定它们的抗生素敏感性。
在阿尔及利亚中部收集了180份来自患有大肠杆菌病病变的肉鸡不同器官（肝脏、脾脏、肺和心脏）的样本。使用常规技术进行分离和鉴定。然后，对这些菌株进行血清分型并针对13种抗生素进行测试。
共分离出156株大肠杆菌。血清分型结果显示，50株属于3种血清型（O1型23株、O2型11株、O78型16株），占分离株的32%。抗菌药敏试验显示，对四环素（94.12%）、氟甲喹（91.5%）、磺胺甲恶唑-甲氧苄啶（88.89%）、恩诺沙星（86.27%）、萘啶酸（85.62%）、氨苄西林（83.01%）和强力霉素（75.81%）呈现高度耐药，对氯霉素（39.22%）和阿莫西林-克拉维酸（43.13%）呈现中度耐药。除3株产超广谱β-内酰胺酶（ESBL）外，所有菌株对头孢噻肟敏感。此外，多重耐药结果显示，所有菌株至少对两种抗生素耐药，66.66%的菌株对至少七种抗生素耐药。
抗生素耐药性继续以惊人的速度上升，ESBL的出现被认为是对公共卫生的一种威胁。
