School of Liquor and Food Engineering, Guizhou University, Guiyang, China.
College of Life Sciences, Guizhou University, Guiyang, China.
J Food Sci. 2023 May;88(5):2009-2022. doi: 10.1111/1750-3841.16570. Epub 2023 Apr 12.
Most colorimetric methods based on nanozymes need to have excellent performance under acidic condition, so they are currently facing some challenges in the field of food hazard detection. Herein, a facile and rapid colorimetric sensor for kanamycin (KAN) detection in foods under neutral condition has been designed using the peroxidase-mimic activity of porous nanozyme like Co O nanodisk. Further investigations showed that the interaction mechanism between porous Co O nanodisk and substrates belongs to a ping-pong model, and the inhibition type of KAN on the peroxidase-mimic activity is noncompetitive inhibition. The constructed sensor has good sensitivity for KAN with the limit of 57 nM, and the color changes can be discerned visually when KAN exceeds 0.5 µM. Besides, the colorimetric sensor obtains excellent recovery results in chicken serum, milk, honey, and pork, which shows that the proposed sensing strategy can provide a rapid and convenient detection method for KAN in foods under neutral condition. PRACTICAL APPLICATION: The established sensing strategy can rapidly distinguish whether KAN residue exceeds the permissible level within 10 min, which meets the requirement for on-site monitoring of antibiotics in foods, and also open up a new idea for other hazards detection under neutral condition.
大多数基于纳米酶的比色法需要在酸性条件下具有优异的性能，因此它们目前在食品安全检测领域面临一些挑战。在此，使用类过氧化物酶活性的多孔纳米酶 CoO 纳米盘设计了一种在中性条件下用于食品中卡那霉素（KAN）检测的简便、快速比色传感器。进一步的研究表明，多孔 CoO 纳米盘与底物之间的相互作用机制属于乒乓模型，KAN 对类过氧化物酶活性的抑制类型是非竞争性抑制。所构建的传感器对 KAN 具有良好的灵敏度，其检出限为 57 nM，当 KAN 超过 0.5 µM 时，颜色变化可以肉眼识别。此外，比色传感器在鸡血清、牛奶、蜂蜜和猪肉中获得了优异的回收率结果，这表明所提出的传感策略可以为中性条件下食品中 KAN 的快速便捷检测提供一种方法。
所建立的传感策略可以在 10 分钟内快速区分 KAN 残留是否超过允许水平，这满足了食品中抗生素现场监测的要求，也为中性条件下其他危害物检测开辟了新思路。
