I. P. Pavlov First St. Petersburg State Medical University, Ministry of Health of the Russian Federation, St. Petersburg, Russia.
I. P. Pavlov Institute of Physiology, Russian Academy of Sciences, St. Petersburg, Russia.
Bull Exp Biol Med. 2023 Mar;174(5):678-680. doi: 10.1007/s10517-023-05768-4. Epub 2023 Apr 13.
The effects of cardiotonic steroids (ouabain and digoxin) on the bone formation were studied using the organotypic tissue culture in combination with confocal microscopy. The expression of α- and α-isoforms of Na,K-ATPase was detected in cells of the bone tissue of 12-day-old chicken embryos. Ouabain in a concentrations 10 M (comparable with its endogenous concentration) can modulate transducer function of Na,K-ATPase and control the growth and proliferation bone tissue cells. Unlike ouabain, digoxin is not involved in the regulation of bone tissue growth in a 12-day-old chicken embryo.
使用器官型组织培养结合共聚焦显微镜研究强心甾体（哇巴因和地高辛）对骨形成的影响。在 12 日龄鸡胚骨组织细胞中检测到α-和α-同工型 Na，K-ATP 酶的表达。浓度为 10 μM 的哇巴因（与内源性浓度相当）可以调节 Na，K-ATP 酶的转导功能，并控制骨组织细胞的生长和增殖。与哇巴因不同，地高辛不参与 12 日龄鸡胚骨组织生长的调节。
