Departamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, CINVESTAV, Ciudad de México 07360, Mexico.
Laboratorio de Biomedicina Molecular, Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero, Chilpancingo 39070, Mexico.
Int J Mol Sci. 2023 Mar 23;24(7):6032. doi: 10.3390/ijms24076032.
miRNAs modulate gene expression and play critical functions as oncomiRs or tumor suppressors. The miR-182-3p is important in chemoresistance and cancer progression in breast, lung, osteosarcoma, and ovarian cancer. However, the role of miR-182-3p in cervical cancer (CC) has not been elucidated.
To analyze the role of miR-182-3p in CC through a comprehensive bioinformatic analysis.
Gene Expression Omnibus (GEO) databases were used for the expression analysis. The mRNA targets of miR-182-3p were identified using miRDB, TargetScanHuman, and miRPathDB. The prediction of island CpG was performed using the MethPrimer program. The transcription factor binding sites in the FLI-1 promoter were identified using ConSite+, Alibaba2, and ALGGEN-PROMO. The protein-protein interaction (PPI) analysis was performed in STRING 11.5.
miR-182-3p was significantly overexpressed in CC patients and has potential as a diagnostic. We identified 330 targets of miR-182-3p including FLI-1, which downregulates its expression in CC. Additionally, the aberrant methylation of the FLI-1 promoter and Ap2a transcription factor could be involved in downregulating FLI1 expression. Finally, we found that FLI-1 is a possible key gene in the immune response in CC.
The miR-182-3p/FLI-1 axis plays a critical role in immune response in CC.
通过综合生物信息学分析来研究 miR-182-3p 在宫颈癌（CC）中的作用。
使用基因表达综合数据库（GEO）进行表达分析。使用 miRDB、TargetScanHuman 和 miRPathDB 来识别 miR-182-3p 的 mRNA 靶标。使用 MethPrimer 程序预测岛 CpG。使用 ConSite+、Alibaba2 和 ALGGEN-PROMO 来识别 FLI-1 启动子中的转录因子结合位点。在 STRING 11.5 中进行蛋白质-蛋白质相互作用（PPI）分析。
miR-182-3p 在 CC 患者中明显过表达，具有作为诊断标志物的潜力。我们鉴定了 330 个 miR-182-3p 的靶标，包括 FLI-1，其在 CC 中下调其表达。此外，AP2A 转录因子的 FLI-1 启动子异常甲基化可能参与下调 FLI1 表达。最后，我们发现 FLI-1 可能是 CC 免疫反应中的关键基因。
miR-182-3p/FLI-1 轴在 CC 中的免疫反应中起关键作用。
