Guo Jia, Su Yuanyuan, Zhang Meng
Stomatological Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052 Henan China.
Cancer Cell Int. 2020 Aug 26;20:407. doi: 10.1186/s12935-020-01501-7. eCollection 2020.
Oral squamous cell carcinoma (OSCC) is a more common cancer in the world. Emerging evidence suggests that circular RNAs (circRNAs) participate in the progression of OSCC. However, the role of circ_0000140 in OSCC is still unknown.
The expression of circ_0000140 and microRNA-182-5p (miR-182-5p) were assessed by quantitative real-time polymerase chain reaction (qRT-PCR). Also, cell proliferation, migration and invasion were measured by colony formation and transwell assays, respectively. Western blot (WB) analysis was used to test the levels of proliferation, metastasis and glycolysis metabolism-related proteins as well as cell division cycle 73 (CDC73) protein. Further, the extracellular acidification rate (ECAR) of cells was detected by the Seahorse XF Extracellular Flux Analyzer. The lactate acid level of cells was tested by Lactate Assay Kit. Moreover, dual-luciferase reporter was used to verify the interaction between miR-182-3p and circ_0000140 or CDC73, and RNA immunoprecipitation (RIP) assay was employed to further confirm the relationship between miR-182-3p and circ_0000140. In addition, mice xenograft models were built to measure the effect of circ_0000140 on OSCC tumor growth in vivo.
Circ_0000140 was lowly expressed in OSCC, and its overexpression hindered proliferation, migration, invasion and glycolysis metabolism in OSCC cells. MiR-182-5p could be sponged by circ_0000140, and its mimic could invert the suppression of circ_0000140 overexpression on OSCC progression. CDC73 could be targeted by miR-182-3p, and its silencing could reverse the inhibition of miR-182-3p inhibitor on OSCC progression. Further, overexpressed circ_0000140 reduced the OSCC tumor growth in vivo.
Circ_0000140 might play an anti-cancer role in OSCC, which provided a novel target for clinical therapy of OSCC.
口腔鳞状细胞癌（OSCC）是全球较为常见的一种癌症。新出现的证据表明，环状RNA（circRNA）参与了OSCC的进展。然而，circ_0000140在OSCC中的作用仍不清楚。
通过定量实时聚合酶链反应（qRT-PCR）评估circ_0000140和微小RNA-182-5p（miR-182-5p）的表达。此外，分别通过集落形成和Transwell实验检测细胞增殖、迁移和侵袭能力。采用蛋白质免疫印迹（WB）分析检测增殖、转移和糖酵解代谢相关蛋白以及细胞分裂周期73（CDC73）蛋白的水平。此外，使用Seahorse XF细胞外流量分析仪检测细胞的细胞外酸化率（ECAR）。通过乳酸检测试剂盒检测细胞的乳酸水平。此外，使用双荧光素酶报告基因验证miR-182-3p与circ_0000140或CDC73之间的相互作用，并采用RNA免疫沉淀（RIP）实验进一步证实miR-182-3p与circ_0000140之间的关系。另外，构建小鼠异种移植模型以检测circ_0000140对OSCC肿瘤在体内生长的影响。
circ_0000140在OSCC中低表达，其过表达可抑制OSCC细胞的增殖、迁移、侵袭和糖酵解代谢。circ_0000140可以吸附miR-182-5p，其模拟物可以逆转circ_0000140过表达对OSCC进展的抑制作用。CDC73可被miR-182-3p靶向，其沉默可逆转miR-182-3p抑制剂对OSCC进展的抑制作用。此外，过表达的circ_0000140可降低OSCC肿瘤在体内的生长。
Circ_0000140可能在OSCC中发挥抗癌作用，为OSCC的临床治疗提供了新的靶点。
