Department for Experimental Oncology, Institute of Oncology and Radiology of Serbia, 11 000 Belgrade, Serbia.
Department for Pharmaceutical Investigations and Development, Institute for Medicinal Plant Research, Dr. Josif Pančić, 11 070 Belgrade, Serbia.
Molecules. 2020 Nov 10;25(22):5233. doi: 10.3390/molecules25225233.
and its secondary metabolites have been shown to have anticancer potential. We performed MTT, scratch, and colony formation assays; analyzed cell cycle phase distribution and doxorubicin uptake and retention with flow cytometry; and detected alterations in the expression of genes involved in the formation of cell-cell interactions and migration using quantitative real-time PCR following treatment of lung adenocarcinoma cells with doxorubicin, extracts, or their combination. MTT assay results suggested strong synergistic effects of the combined treatments, and their application led to an increase in cell numbers in the subG1 phase of the cell cycle. Both extracts were shown to prolong doxorubicin retention time in cancer cells, while the application of doxorubicin/extract combination led to a decrease in expression. Furthermore, cells treated with doxorubicin/extract combinations were shown to have lower migratory and colony formation potentials than untreated cells or cells treated with doxorubicin alone. The obtained results suggest that nontoxic extracts can enhance the activity of doxorubicin, thus potentially allowing the application of lower doxorubicin doses in vivo, which may decrease its toxic effects in normal tissues.
并且其次生代谢产物已被证明具有抗癌潜力。我们通过 MTT、划痕和集落形成实验；通过流式细胞术分析细胞周期相分布和阿霉素摄取和保留；并在使用阿霉素、提取物或它们的组合处理肺腺癌细胞后，使用实时定量 PCR 检测参与细胞-细胞相互作用和迁移形成的基因的表达变化。MTT 检测结果表明联合处理具有很强的协同作用，它们的应用导致细胞周期中 subG1 期的细胞数量增加。两种提取物都被证明能延长阿霉素在癌细胞中的保留时间，而阿霉素/提取物组合的应用导致 表达下降。此外，与未处理细胞或单独用阿霉素处理的细胞相比，用阿霉素/提取物组合处理的细胞表现出较低的迁移和集落形成能力。研究结果表明，非毒性的提取物可以增强阿霉素的活性，从而可能允许在体内应用较低剂量的阿霉素，这可能会降低其在正常组织中的毒性作用。
