Department of Chemical Sciences, University of Naples Federico II, Via Cinthia 4, 80126 Naples, Italy.
Laboratory of Foodomics, Institute of Food Science Research, CIAL, CSIC, Nicolás Cabrera 9, 28049 Madrid, Spain.
Molecules. 2023 Mar 31;28(7):3144. doi: 10.3390/molecules28073144.
Microalgal biomass represents a very interesting biological feedstock to be converted into several high-value products in a biorefinery approach. In this study, the cyanobacterium sp. PCC6803 was used to obtain different classes of molecules: proteins, carotenoids and lipids by using a cascade approach. In particular, the protein extract showed a selective cytotoxicity towards cancer cells, whereas carotenoids were found to be active as antioxidants both in vitro and on a cell-based model. Finally, for the first time, lipids were recovered from biomass as the last class of molecules and were successfully used as an alternative substrate for the production of polyhydroxyalkanoate (PHA) by the native PHA producer . Taken together, our results lead to a significant increase in the valorization of sp. PCC6803 biomass, thus allowing a possible offsetting of the process costs.
微藻生物质是一种非常有趣的生物原料，可以通过生物炼制方法转化为多种高价值产品。在这项研究中，使用蓝藻 sp. PCC6803 通过级联方法获得不同类别的分子：蛋白质、类胡萝卜素和脂质。特别是，蛋白质提取物对癌细胞表现出选择性细胞毒性，而类胡萝卜素被发现具有体外和基于细胞模型的抗氧化活性。最后，首次从生物质中回收脂质作为最后一类分子，并成功用作天然聚羟基脂肪酸酯 (PHA) 生产者生产 PHA 的替代底物。总之，我们的结果导致 sp. PCC6803 生物质的增值显著增加，从而有可能抵消工艺成本。
