Nilash Amid B, Jahanbani Jahanfar, Jolehar Maryam
General Dentist, Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
Adv Biomed Res. 2023 Feb 25;12:53. doi: 10.4103/abr.abr_305_21. eCollection 2023.
Considering the global prevalence of cancers and the complications of common cancer treatments, there is growing interest in using medicinal herbs to complement cancer treatments and reduce treatment's side effects. Therefore, we investigate the effect of the extract of Nasturtium on the viability of oral cancer cells.
In this experimental study, we prepared aqueous extract from Nasturtium leaves and human oral cancer cells (OCC-24) and normal fibroblast cells (HF2FF cell line) from a cell bank. Then the toxic effect of different concentrations of the extract on cell viability after 24-48 hours of exposure was investigated with the methylthiazol tetrazolium assay. Ultimately, the optical density was measured at 570 nm by an Elisa Reader. Analysis of inhibitory concentration 50 (IC50) was also performed. The data were analyzed by paired Student's -test and one-way analysis of variance.
Data showed that the extract had statistically significant anticancer effects in concentrations above 0.125 mg/ml for 24-hour exposure and in concentrations above 0.5 mg/ml for 48-hour exposure (p-value <0.05). Also, this extract had an adverse effect on the viability of normal cells; however, this effect occurred in high doses of the extract (p-value <0.05). Analysis of IC50 criteria indicates that with increasing time, a higher concentration of the extract is required to inhibit the viability of cancer cells.
Because of the results, this aqueous extract can be suggested as a potential therapeutic agent in oral cancer. The best concentration of the extract was found to be 1 mg/ml.
鉴于癌症在全球的患病率以及常见癌症治疗的并发症，人们越来越有兴趣使用草药来辅助癌症治疗并减轻治疗的副作用。因此，我们研究了旱金莲提取物对口腔癌细胞活力的影响。
在这项实验研究中，我们从细胞库中获取了旱金莲叶子的水提取物、人类口腔癌细胞（OCC - 24）和正常成纤维细胞（HF2FF细胞系）。然后，通过甲基噻唑四氮唑法研究了不同浓度提取物在暴露24 - 48小时后对细胞活力的毒性作用。最终，使用酶标仪在570nm处测量光密度。还进行了半数抑制浓度（IC50）分析。数据采用配对t检验和单因素方差分析。
数据显示，对于24小时暴露，浓度高于0.125mg/ml时提取物具有统计学显著的抗癌作用；对于48小时暴露，浓度高于0.5mg/ml时具有显著抗癌作用（p值<0.05）。此外，该提取物对正常细胞的活力有不良影响；然而，这种影响发生在高剂量提取物时（p值<0.05）。IC50标准分析表明，随着时间增加，需要更高浓度的提取物来抑制癌细胞的活力。
基于这些结果，这种水提取物可被建议作为口腔癌的一种潜在治疗剂。提取物的最佳浓度为1mg/ml。
