Biosystem and Bioengineering Program, University of Science and Technology (UST), Daejeon, 34141, Republic of Korea.
Plant System Engineering Research Center, Korean Research Institute of Bioscience and Biotechnology, Daejeon, 34141, Republic of Korea.
Virol J. 2024 Apr 15;21(1):86. doi: 10.1186/s12985-024-02361-7.
Viruses have notable effects on agroecosystems, wherein they can adversely affect plant health and cause problems (e.g., increased biosecurity risks and economic losses). However, our knowledge of their diversity and interactions with specific host plants in ecosystems remains limited. To enhance our understanding of the roles that viruses play in agroecosystems, comprehensive analyses of the viromes of a wide range of plants are essential. High-throughput sequencing (HTS) techniques are useful for conducting impartial and unbiased investigations of plant viromes, ultimately forming a basis for generating further biological and ecological insights. This study was conducted to thoroughly characterize the viral community dynamics in individual plants.
An HTS-based virome analysis in conjunction with proximity sampling and a tripartite network analysis were performed to investigate the viral diversity in chunkung (Cnidium officinale) plants. We identified 61 distinct chunkung plant-associated viruses (27 DNA and 34 RNA viruses) from 21 known genera and 6 unclassified genera in 14 known viral families. Notably, 12 persistent viruses (7 DNA and 5 RNA viruses) were exclusive to dwarfed chunkung plants. The detection of viruses from the families Partitiviridae, Picobirnaviridae, and Spinareoviridae only in the dwarfed plants suggested that they may contribute to the observed dwarfism. The co-infection of chunkung by multiple viruses is indicative of a dynamic and interactive viral ecosystem with significant sequence variability and evidence of recombination.
We revealed the viral community involved in chunkung. Our findings suggest that chunkung serves as a significant reservoir for a variety of plant viruses. Moreover, the co-infection rate of individual plants was unexpectedly high. Future research will need to elucidate the mechanisms enabling several dozen viruses to co-exist in chunkung. Nevertheless, the important insights into the chunkung virome generated in this study may be relevant to developing effective plant viral disease management and control strategies.
病毒对农业生态系统有显著影响，它们会对植物健康造成负面影响，并引发各种问题（例如增加生物安全风险和经济损失）。然而，我们对其在生态系统中与特定宿主植物的多样性和相互作用的了解仍然有限。为了增强我们对病毒在农业生态系统中所扮演角色的理解，对广泛植物的病毒组进行全面分析至关重要。高通量测序（HTS）技术可用于公正、无偏地调查植物病毒组，最终为进一步的生物学和生态学研究提供基础。本研究旨在全面描述单个植物中的病毒群落动态。
本研究采用 HTS 为基础的病毒组分析，结合临近采样和三分网络分析，对 chunkung（Cnidium officinale）植物中的病毒多样性进行了研究。我们从 21 个已知属和 6 个未分类属的 14 个已知病毒科中鉴定出 61 种不同的 chunkung 植物相关病毒（27 种 DNA 和 34 种 RNA 病毒）。值得注意的是，12 种持续性病毒（7 种 DNA 和 5 种 RNA 病毒）仅存在于矮化 chunkung 植物中。仅在矮化植物中检测到来自 Partitiviridae、Picobirnaviridae 和 Spinareoviridae 家族的病毒，表明它们可能导致了观察到的矮化现象。多个病毒在 chunkung 中的共同感染表明存在一个动态交互的病毒生态系统，具有显著的序列变异性和重组证据。
我们揭示了与 chunkung 相关的病毒群落。研究结果表明，chunkung 是多种植物病毒的重要储存库。此外，单个植物的共同感染率出人意料地高。未来的研究需要阐明使数十种病毒能够在 chunkung 中共存的机制。尽管如此，本研究中对 chunkung 病毒组的重要发现可能有助于制定有效的植物病毒病管理和控制策略。
