School of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China.
School of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China.
Int J Biol Macromol. 2023 Jun 15;240:124435. doi: 10.1016/j.ijbiomac.2023.124435. Epub 2023 Apr 14.
Normal intestinal epithelial barrier function plays a key role in the prevention of many diseases such as infectious enteritis, inflammatory bowel disease, obesity, etc. In this study, three novel acidic polysaccharides ZY-2, ZY-3 and ZY-4 were isolated from sour jujube (Ziziphus jujuba Mill. var. Spinosa) seeds and purified by DEAE Sephrose Fast Flow gel. The molecular weight of ZY-2, ZY-3 and ZY-4 was 7.76 kDa, 10.71 kDa and 8.31 kDa respectively, mainly composed of different proportions of mannose, rhamnose, glucose, glucuronic acid, galacturonic acid, galactose, xylose and arabinose. H NMR and Congo red experiment results showed that the three polysaccharides mainly contained both α-type and β-type glycosidic bonds with obvious triple helix structural traits. The polysaccharides could up-regulate the expression levels of occludin and ZO-1 in LPS-induced inflammation Caco-2 cells, and reduce IL-6, IL-8, IL-1β and TNF-α significantly. In conclusion, the acidic polysaccharides from sour jujube seeds exhibited great potential in protection intestinal epithelial barrier function through anti-inflammatory effects.
正常的肠道上皮屏障功能在预防许多疾病方面起着关键作用，如感染性肠炎、炎症性肠病、肥胖等。在这项研究中，从酸枣(Ziziphus jujuba Mill. var. Spinosa)种子中分离并纯化了三种新型酸性多糖 ZY-2、ZY-3 和 ZY-4，通过 DEAE Sephrose Fast Flow 凝胶。ZY-2、ZY-3 和 ZY-4 的分子量分别为 7.76 kDa、10.71 kDa 和 8.31 kDa，主要由不同比例的甘露糖、鼠李糖、葡萄糖、葡萄糖醛酸、半乳糖醛酸、半乳糖、木糖和阿拉伯糖组成。1 H NMR 和刚果红实验结果表明，这三种多糖主要含有α型和β型糖苷键，具有明显的三螺旋结构特征。多糖可上调 LPS 诱导的炎症 Caco-2 细胞中 occludin 和 ZO-1 的表达水平，并显著降低 IL-6、IL-8、IL-1β 和 TNF-α。综上所述，酸枣种子中的酸性多糖通过抗炎作用显示出保护肠道上皮屏障功能的巨大潜力。
