Naeem Muhammad, Amaro-Estrada Itzel, Taqadus Asia, Swelum Ayman A, Alqhtani Abdulmohsen H, Asif Muhammad, Sajid Muhammad, Khan Asmat Ullah, Tariq Aliza, Anjum Summaya, Khan Adil, Iqbal Furhan
Institute of Zoology, Bahauddin Zakariya University, Multan, Pakistan.
Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP), Jiutepec, Morelos, Mexico.
Front Vet Sci. 2023 Mar 29;10:1096418. doi: 10.3389/fvets.2023.1096418. eCollection 2023.
Majority of Pakistani population lives in rural areas and raising animals, especially the small ruminants, is their primary source of income. is known to infect small ruminants globally and causing significant economic losses to livestock owners, however prevalence of has been least investigated from Pakistan despite having a huge sheep population.
The present study was conducted from June 2021 till December 2021 to report the PCR based prevalence of in the blood samples of sheep ( = 239) that were collected from District Dera Ghazi Khan in Pakistan.
Out of 239 samples, 30 (12.5%) amplified a 347 bp fragment specific for the gene of . Represented partial gene sequences were confirmed by Sanger sequencing and deposited to GenBank (OP620757-59). None of the studied epidemiological factors (age, sex, breed, size of herd, dogs with herd, and composition of herd) showed an association ( > 0.05) with the infection in enrolled sheep. Analysis of the amplified partial sequence of revealed that this gene is highly conserved as all three sequences were identical and phylogenetically resembled with the sequences amplified from small ruminants in China, Kenya, and Germany, Turkey, Portugal, Tunisia and India. In conclusion, for the first time, we are reporting a moderate prevalence of prevalence in Pakistani sheep and this data will help in developing the integrated control policies against this newly reported tick-borne disease that is infecting our sheep breeds.
大多数巴基斯坦人口生活在农村地区，饲养动物，尤其是小型反刍动物，是他们的主要收入来源。已知[病原体名称]在全球范围内感染小型反刍动物，并给牲畜所有者造成重大经济损失，然而，尽管巴基斯坦拥有庞大的绵羊种群，但对[病原体名称]的流行情况研究最少。
本研究于2021年6月至2021年12月进行，以报告从巴基斯坦德拉加齐汗地区采集的绵羊（n = 239）血样中基于PCR的[病原体名称]流行情况。
在239个样本中，30个（12.5%）扩增出了一段347 bp的特定于[病原体名称]基因的片段。所代表的部分[病原体名称]基因序列经桑格测序确认，并提交至GenBank（OP620757 - 59）。所研究的流行病学因素（年龄、性别、品种、畜群规模、带犬畜群以及畜群组成）均未显示与纳入研究的绵羊感染[病原体名称]存在关联（P > 0.05）。对扩增的[病原体名称]部分序列分析表明，该基因高度保守，因为所有三个序列均相同，并且在系统发育上与从中国、肯尼亚、德国、土耳其、葡萄牙、突尼斯和印度的小型反刍动物中扩增的[病原体名称]序列相似。总之，我们首次报告了巴基斯坦绵羊中[病原体名称]的中等流行率，这些数据将有助于制定针对这种新报告的感染我们绵羊品种的蜱传疾病的综合防控政策。
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