Cepheid, Sunnyvale, California, USA.
Danaher Diagnostics, Washington, DC, USA.
J Clin Invest. 2023 Apr 17;133(8):e169242. doi: 10.1172/JCI169242.
Optimal management of lower respiratory tract infection relies on distinguishing infectious from noninfectious etiologies and identifying the microbiologic cause if applicable. This process is complicated by overlapping clinical symptoms and the colonizing lung microbiota. In a recent issue of the JCI, Mick, Tsitsiklis, and colleagues apply RNA-Seq to tracheal aspirates from critically ill children and demonstrate how integration of the host response with microbial identification results in a harmonious and accurate diagnostic classifier. Though promising, there are numerous barriers to realizing a combined host and pathogen diagnostic.
下呼吸道感染的最佳管理依赖于区分感染性和非感染性病因，并在适用的情况下确定微生物病因。这一过程受到重叠的临床症状和定植于肺部的微生物组的影响而变得复杂。在最近一期的 JCI 中，Mick、Tsitsiklis 及其同事应用 RNA-Seq 分析了危重症患儿的气管吸出物，并展示了如何将宿主反应与微生物鉴定结果相结合，从而得到一个和谐且准确的诊断分类器。尽管前景广阔，但要实现宿主和病原体的联合诊断，仍存在许多障碍。
