Silva Saimon M, Li Miaosi, Mendes Alexandre Xavier, Moulton Simon E
Universal Biosensors, Rowville, Victoria 3178, Australia.
Aikenhead Centre for Medical Discovery, St Vincent's Hospital Melbourne, Melbourne, Victoria 3065, Australia.
Analyst. 2023 May 2;148(9):1930-1938. doi: 10.1039/d3an00341h.
The creation of reagentless protein-based biosensors that are capable of monitoring molecular analytes directly in bodily fluids could revolutionize our understanding of biology and personalized health monitoring. The limited number of molecular sensors that are currently available in the market depends on the specific enzymatic or chemical reactivity of their target analytes and therefore are not applicable to many relevant biomarkers. Aiming to overcome this limited molecular sensing generality, a new class of reagentless protein-based electrochemical sensors has been introduced for the direct measurements of biomarkers in unprocessed biological fluids. This mini-review will discuss the most recent cutting-edge discoveries for the development of electroanalytical modular biosensors, where all the sensors' components are integrated into a self-sufficient sensor allowing hence its autonomous functionality.
能够直接在体液中监测分子分析物的无试剂蛋白质生物传感器的出现，可能会彻底改变我们对生物学和个性化健康监测的理解。目前市场上可用的分子传感器数量有限，这取决于其目标分析物的特定酶促或化学反应性，因此不适用于许多相关生物标志物。为了克服这种有限的分子传感通用性，已引入了一类新型的无试剂蛋白质基电化学传感器，用于直接测量未处理生物流体中的生物标志物。本综述将讨论电分析模块化生物传感器开发的最新前沿发现，其中所有传感器组件都集成到一个自给自足的传感器中，从而实现其自主功能。
