Department of General Surgery, Chungnam National University Hospital, Daejeon, 35015, Republic of Korea.
Department of Plastic and Reconstructive Surgery, Chungnam National University Hospital, Daejeon, 35015, Republic of Korea.
Aesthetic Plast Surg. 2023 Dec;47(6):2833-2840. doi: 10.1007/s00266-023-03353-8. Epub 2023 Apr 17.
Human acellular dermal matrix (hADM) has found applications in a variety of settings, particularly in breast surgery. The most common hADM is a sheet. Recently, an injectable hADM has been introduced; we compared the biocompatibility and long-term structural integrity of, an injectable hADM and a sheet-type hADM in mice.
An injectable hADM (experimental group) and a sheet-type hADM (control group) were implanted into sub-panniculus pockets on the backs of 50 mice. The animals were sacrificed 2, 4, 8, 12, or 24 weeks later and the hADMs and surrounding tissues were recovered and stained for histopathological analyses. The microscopic endpoints included the thickness of the hADM and capsule around the hADM, and the extents of fibroblast proliferation and neovascularization.
No animal developed a complication or infection. The capsule was significantly thinner in the experimental than the control group. There were no significant differences between groups in the hADM thickness. Microscopically, the fibroblast density inside the hADM was significantly higher in the experimental group. The fibroblasts inside of the hADM lay significantly deeper in the experimental group. Similarly, the experimental group exhibited significantly deeper microvessels inside the hADM.
The injectable hADM had a thinner capsule thickness (more biocompatible), than the sheet-type hADM. It maintained its thickness as well as the sheet-type hADM and had a more fibroblast proliferation and neovascularization. This means the tissue incorporation and long-term structural integrity of the injectable hADM may be as good as or better than that of the sheet-type hADM.
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人源去细胞真皮基质（hADM）已在多种情况下得到应用，特别是在乳房手术中。最常见的 hADM 是片状的。最近，一种可注射的 hADM 已经问世；我们比较了可注射的 hADM 和片状 hADM 在小鼠体内的生物相容性和长期结构完整性。
将可注射的 hADM（实验组）和片状的 hADM（对照组）植入 50 只小鼠背部的皮下囊袋中。2、4、8、12 或 24 周后处死动物，取出 hADM 和周围组织进行组织病理学分析。微观终点包括 hADM 及其周围包膜的厚度、成纤维细胞增殖和新生血管形成的程度。
没有动物发生并发症或感染。实验组的包膜明显比对照组薄。两组间 hADM 的厚度无显著差异。镜下，实验组 hADM 内的成纤维细胞密度明显高于对照组。实验组 hADM 内的成纤维细胞位置更深。同样，实验组 hADM 内的微血管也更深。
与片状 hADM 相比，可注射的 hADM 包膜厚度更薄（生物相容性更好）。它保持了与片状 hADM 相同的厚度，并且有更多的成纤维细胞增殖和新生血管形成。这意味着可注射 hADM 的组织整合和长期结构完整性可能与片状 hADM 一样好，甚至更好。
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