Jahanshiri Zahra, Manifar Soheila, Hatami Farahnaz
Department of Mycology, Pasteur Institute of Iran, Tehran, Iran.
Department of Oral Medicine, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran.
Iran J Microbiol. 2023 Feb;15(1):163-173. doi: 10.18502/ijm.v15i1.11933.
is one of the major non- species causing nosocomial infection. There is limited data about mechanisms of azole-resistance and virulence factors of . This study was designed to investigate molecular mechanism of azole-resistance and major virulence factors of isolated from oropharyngeal candidiasis in head and neck cancer patients.
After collecting 38 clinical isolates, antifungal susceptibility pattern and the expression levels of and were evaluated. Moreover, proteinase and phospholipase activity and biofilm formation of the isolates were investigated as virulence factors.
We detected fluconazole resistance in 7 isolates. The expression levels of and were increased respectively. Protease activity and biofilm formation were seen in all isolates. Five isolates did not exhibit phospholipase activity.
Taken together, the overexpressions of and genes were found in fluconazole resistant , isolated from oropharyngeal candidiasis patients. Also, voriconazole was an effective antifungal against isolates. The observed high protease enzyme activity and biofilm formation suggested strong pathogenicity of these isolates.
是引起医院感染的主要非 菌种之一。关于 唑类耐药机制和 毒力因子的数据有限。本研究旨在调查从头颈癌患者口咽念珠菌病中分离出的 的唑类耐药分子机制和主要毒力因子。
收集38株 临床分离株后，评估其抗真菌药敏模式以及 和 的表达水平。此外，还研究了分离株的蛋白酶和磷脂酶活性以及生物膜形成作为毒力因子。
我们在7株分离株中检测到氟康唑耐药。 和 的表达水平分别升高。所有分离株均可见蛋白酶活性和生物膜形成。5株分离株未表现出磷脂酶活性。
总体而言，在从口咽念珠菌病患者中分离出的氟康唑耐药 中发现了 和 基因的过表达。此外，伏立康唑对 分离株是一种有效的抗真菌药物。观察到的高蛋白酶活性和生物膜形成表明这些分离株具有很强的致病性。
