Molecular Biology and Genetics Laboratory (LABIOGENE), University of Joseph Ki ZERBO, BP 7021, Ouagadougou 03, Burkina Faso.
Research Institute for Health Sciences (IRSS), 03 BP 7047, Ouagadougou 03, Burkina Faso.
BMC Infect Dis. 2022 Aug 8;22(1):678. doi: 10.1186/s12879-022-07619-5.
Vulvovaginal candidiasis is an important cause of morbidity among women due to Candida species. In the last decades, resistance to azoles, first-line antifungals has increased. One molecular mechanism of azole resistance by Candida involves mutations in the ERG11 gene encoding lanosterol 14-α-demethylase, the target enzyme. This study was conducted to identify the clinical Candida species associated in vulvovaginal candidiasis; to determine the rate of antifungal resistance among Candida albicans isolates and to determine mutated ERG11 gene at Saint Camille Hospital in Ouagadougou, Burkina Faso.
Antifungals susceptibility were performed using Kirby-Bauer disk diffusion method. ERG11 gene was detected using conventional PCR in C. albicans isolates resistant to at least one azole.
Out of 262 clinical strains isolated, C. albicans accounted for 59.90%, followed by Candida glabrata 27.86%, Candida famata 7.25%, Candida tropicalis 3.05% and Saccharomyces cerevisiae 1.91%. Resistance rate of fluconazole to C. albicans was 59.54%. ERG11 gene was found in 9.79% of 92 C. albicans strains resistant to azoles.
This detection of mutated ERG11 gene in C. albicans is the first in Burkina Faso and may be a cause of azole resistance in recurrent Candida vulvovaginitis.
外阴阴道假丝酵母菌病是由念珠菌属引起的女性发病率较高的重要疾病。在过去几十年中，抗真菌药物唑类药物的耐药性增加。念珠菌对唑类药物耐药的一种分子机制涉及编码羊毛甾醇 14-α-脱甲基酶（靶酶）的 ERG11 基因突变。本研究旨在鉴定外阴阴道假丝酵母菌病相关的临床念珠菌种；确定布基纳法索瓦加杜古圣卡米尔医院分离的白假丝酵母菌对氟康唑的耐药率，并确定突变的 ERG11 基因。
采用 Kirby-Bauer 纸片扩散法进行抗真菌药敏试验。用常规 PCR 检测对至少一种唑类药物耐药的白假丝酵母菌中 ERG11 基因。
从 262 株临床分离株中，白假丝酵母菌占 59.90%，其次是光滑假丝酵母菌 27.86%、近平滑假丝酵母菌 7.25%、热带假丝酵母菌 3.05%和酿酒酵母 1.91%。氟康唑对白假丝酵母菌的耐药率为 59.54%。在对唑类药物耐药的 92 株白假丝酵母菌中，发现 ERG11 基因突变基因占 9.79%。
本研究首次在布基纳法索检测到白假丝酵母菌中突变的 ERG11 基因，可能是复发性外阴阴道假丝酵母菌病唑类耐药的原因。
