Department of Histology and Embryology, School of Medicine, Bahcesehir University, Istanbul, Turkey.
Department of Histology and Embryology, Cerrahpasa Faculty of Medicine, Istanbul University-Cerrahpasa, Istanbul, Turkey.
J Histochem Cytochem. 2023 Apr;71(4):211-225. doi: 10.1369/00221554231170662. Epub 2023 Apr 18.
Preeclampsia is a complication of pregnancy that affects 3-5% of pregnancies and is one of the major causes of maternal/neonatal mortality and morbidities worldwide. We aimed to investigate the distribution of Foxp3+ regulatory T-cells and CD68+ Hofbauer cells in the placenta of preeclamptic and healthy pregnant women with a special focus on correlating these findings with placental histology. Decidua and chorionic villi of the placenta obtained from healthy and preeclamptic pregnancies were evaluated in full-thickness sections. Sections were stained with hematoxylin and eosin and Masson's trichrome and immunostained for Foxp3 and CD68 for histological analyses. The total histomorphological score for placentas was found to be higher in preeclamptic placentas than that in the controls. The CD68 immunoreactivity was higher in the chorionic villi of preeclamptic placentas than that in the controls. The immunoreactivity of Foxp3 was found widely distributed within the decidua in both the groups and did not differ significantly. Interestingly, Foxp3 immunoreactivity in the chorionic villi was found mainly in the villous core and, to a lesser extent, in the syncytiotrophoblasts. We found no significant relation between Foxp3 expressions and morphological changes observed in preeclamptic placentas. Although extensive research is being carried out regarding the pathophysiology of preeclampsia, the findings are still controversial.
子痫前期是一种妊娠并发症，影响 3-5%的妊娠，是全球孕产妇/新生儿死亡和发病的主要原因之一。我们旨在研究子痫前期和健康孕妇胎盘组织中 Foxp3+调节性 T 细胞和 CD68+Hofbauer 细胞的分布，并特别关注这些发现与胎盘组织学的相关性。对来自健康和子痫前期妊娠的胎盘蜕膜和绒毛进行全层切片评估。用苏木精和伊红、Masson 三色和 Foxp3 和 CD68 免疫染色进行组织学分析。与对照组相比，子痫前期胎盘的总组织形态学评分更高。子痫前期胎盘的绒毛 CD68 免疫反应性高于对照组。Foxp3 的免疫反应性在两组蜕膜中广泛分布，无显著差异。有趣的是，绒毛中的 Foxp3 免疫反应性主要位于绒毛核心，其次是合体滋养层。我们没有发现 Foxp3 表达与子痫前期胎盘观察到的形态变化之间有显著关系。尽管针对子痫前期的病理生理学进行了广泛的研究，但结果仍然存在争议。
