Hussein Sumaiah I, Al-Yasiri Amal Y, Hassan Heba F, Kashman Basim M, Azeez Rasha A
Department of Basic Sciences, College of Dentistry, University of Baghdad, Baghdad, Iraq.
National Cancer Research Center, University of Baghdad, Baghdad, Iraq.
Lasers Med Sci. 2023 Apr 19;38(1):106. doi: 10.1007/s10103-023-03765-7.
The current study was performed to investigate the treatment of tumors with gold nanoparticles, laser, and photodynamic therapy (PDT) by using an immunohistochemistry method and to investigate the expression of FOXP1 in infected mice with mammary adenocarcinoma whether it can be used as an indicator to estimate the recovery of tissues from cancer disease. Twenty-five albino female mice were used in this research; they were divided into five groups, four groups were infected with mammary adenocarcinoma, and then three of them were treated with gold nanoparticles, laser, and PDT, respectively, while the fourth group was left without any treatment and represents the positive control, and the fifth group (normal mice) represents the negative control. Tissue sections were taken from different groups of mice in order to estimate FOXP1 expression in infected mice by using an immunohistochemistry assay. FOXP1 expression was higher in the tumor and kidney tissues of the mice treated with PDT than that in mice treated with either gold nanoparticles or laser alone. Also, in the group of mice treated with laser, FOXP1 expression was higher than the expression in mice which were treated with gold nanoparticles but lower than that in mice which were treated with PDT. FOXP1 can be used as a biomarker for the prognosis outcome of breast and other solid tumors, as well as it considers a key tumor suppressor. PDT is the best choice to treat cancer in comparison to using either gold nanoparticles or the laser separately.
本研究旨在通过免疫组织化学方法研究金纳米颗粒、激光和光动力疗法（PDT）对肿瘤的治疗效果，并研究乳腺腺癌感染小鼠中FOXP1的表达情况，探讨其是否可作为评估癌症疾病组织恢复情况的指标。本研究使用了25只白化雌性小鼠，将其分为五组，其中四组感染乳腺腺癌，然后分别对其中三组进行金纳米颗粒、激光和PDT治疗，第四组不进行任何治疗作为阳性对照，第五组（正常小鼠）作为阴性对照。取不同组小鼠的组织切片，通过免疫组织化学分析评估感染小鼠中FOXP1的表达。接受PDT治疗的小鼠肿瘤组织和肾脏组织中FOXP1的表达高于单独接受金纳米颗粒或激光治疗的小鼠。此外，在接受激光治疗的小鼠组中，FOXP1的表达高于接受金纳米颗粒治疗的小鼠，但低于接受PDT治疗的小鼠。FOXP1可作为乳腺癌和其他实体瘤预后结果的生物标志物，也是一种关键的肿瘤抑制因子。与单独使用金纳米颗粒或激光相比，PDT是治疗癌症的最佳选择。
