黑胸切叶蜂的全基因组（膜翅目：切叶蜂科）：对玻璃叶蜂比较基因组学的重要一步。

The Whole Genome of Colletes collaris (Hymenoptera: Colletidae): An Important Step in Comparative Genomics of Cellophane Bees.

机构信息

Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.

Environmental Science Training Center, Federal University of Southern Bahia, Porto Seguro, Brazil.

出版信息

Genome Biol Evol. 2023 May 5;15(5). doi: 10.1093/gbe/evad062.

DOI:10.1093/gbe/evad062

PMID:37075227

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10159585/

Abstract

Gaining knowledge on bees is of the utmost importance due to the paramount role that they play in angiosperm pollination. Herein, we provide the first genome assembly of Colletes collaris, a pan-Eurasian cellophane bee. We sequenced 50.53 Gbp of long-read data plus 57.36 Gbp of short-read data in Oxford Nanopore Technologies and Illumina platforms, respectively. The genome assembly consisted of 374.75 Mbp distributed across 374 contigs, with L50 and N50 of 9 and 8.96 Mbp, respectively. We predicted the genome to comprise 20,399 protein-coding genes, 467,947 repeats, and 4,315 non-coding RNA genes. The transcriptome and mitochondrial genome of the species were also assembled. Gene family analysis with 15 insect species identified 14,417 families, 9,517 of them found in C. collaris. A dated phylogenomic analysis revealed high numbers of orthogroups experiencing rapid evolution within Colletes.

摘要

由于蜜蜂在被子植物授粉中起着至关重要的作用，因此了解蜜蜂至关重要。本文提供了欧洲 - 亚洲细胞纸蜂 Colletes collaris 的首个基因组组装。我们分别在牛津纳米孔技术和 Illumina 平台上测序了 50.53 Gbp 的长读数据和 57.36 Gbp 的短读数据。基因组组装由 374.75 Mbp 组成，分布在 374 个 contigs 中，L50 和 N50 分别为 9 和 8.96 Mbp。我们预测该基因组包含 20399 个编码蛋白的基因、467947 个重复序列和 4315 个非编码 RNA 基因。该物种的转录组和线粒体基因组也被组装。与 15 种昆虫的基因家族分析确定了 14417 个家族，其中 9517 个家族存在于 C. collaris 中。经过时间的系统发育基因组分析显示，在 Colletes 中，有大量经历快速进化的直系同源基因。